Akduman, EEcemis, TSagol, SÇiçek, CVatansever, SÖzbakkaloglu, B2024-07-182024-07-182146-23721309-9566http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/4844Objective: The aim of this study was to compare the reliability of hybridization and direct fluorescent antibody tests for the diagnosis of genital Chlamydia trachomatis infections. Material and Methods: Cervical specimens from 100 infertile women were evaluated with hybridizasyon, direct fluorescent antibody tests, and cell culture method known as a gold standard, and also sensitivity, specificity, positive and negative predictive values of the three methods were compared. Results: C. trachomatis was isolated in cell cultures of 11 samples. Seven and 19 positive samples were found respectively through direct fluorescent antibody, and hybridization tests identified 7, and 19 positive samples, respectively. Direct fluorescent antibody tests demonstrated 54.5 % sensitivity, 98 % specificity, 47.4 % positive, and 94.6 % negative predictive value, while the corresponding percentages for hybridization tests were 81.8, 88.8, 47.4, and 97.5 %, respectively. Conclusion: It was concluded that a single method has not sufficient sensitivity and specificity for the establishment of a definitive diagnosis C. trachomatis infection, and the results should be confirmed by other methods the hybridization method being more rapid and effective than the other methods.TurkishArticle