Meltem BAYRAKTARElmira NAZİRİFatih Karabeyİsmail Hakkı AKGÜNErdal BEDİRBarbel Röck OKUYUCUAynur GÜREL2024-07-242024-07-242018http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/23516Stevia rebaudiana Bertoni, which is an important plant for the foodand health sector, contains calorie-free natural sweet-tasting steviol glycosides(SGs). In the present study, the effects of different elicitors [methyl jasmonate(MeJA), salicylic acid (SA), or chitosan (CHI)] on the in vitro production ofstevioside and rebaudioside A were carried out. For this purpose, 3-week-old invitro plantlets were transferred into 250 mL flasks containing liquid woody plantmedium (WPM) supplemented with MeJA, SA, or CHI at differentconcentrations (0, 50, 100, or 200 µM), and were exposed to these elicitors for 2weeks. A HPLC method was developed to quantify the aforementioned SGs inthe cultivated plantlets and all of the elicitor types and concentrations resulted inan increase in stevioside production ranged between 2.87 mg/g dry weight (DW)(Control) and 50.07 mg/g DW (100 µM MeJA). The highest number of shoot,node, leaf, leaf length, and biomass accumulation and shoot length were observedwith application of 100 µM CHI and control, respectively. The present findingsopen new perspectives for increasing the stevioside production using a planttissue culture system.engAraştırma Makalesi10.21448/ijsm.496724