Karlitepe A.Kabadayi H.Vatansever S.Gurdal M.Gunduz C.Ercan G.2024-07-222024-07-22201718129269http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15370Aim: The aim of this study is to investigate the effects of miR150 transfection on NK-like cells differentiated from adipose tissue derived mesenchymal stem cells (AD-MSCs). Methods: NK-like cells were differentiated from AD-MSCs and activated by miR150 transfection. Transfected/non-Transfected NK-like cells were characterized by immunohistochemical and RTPCR analyzes. Apoptotic efficiency of the transfected/non-Transfected NK-like cells on pancreatic cancer cells PANC1 were determined by TUNEL and RT-PCR. Results: In miR150-Transfected cells, the increased expression of NK cell-specific genes such as GKMB, KIR2DL2, CD16, CD56, NKG2D, NKp46 and increased immunoreactivity of NK cell-specific surface marker CD314 (NKG2D) were evident. TUNEL assays showed that NK-like cells with/without transfection induced apoptosis in PANC1 cells in the same manner. The decrease in oncogene expression and the increase in the tumor suppressor gene expression in PANC1 cells upon co-culture with NK-like cells differentiated from AD-MSCs were more prominent following miRNA150 transfection. Conclusion: It was shown in vitro that NK-like cells could be obtained by differentiation from AD-MSCs and their efficiency could be increased via miR150 transfection. The results are encouraging for further clinical studies in improvement of immunotherapeutic approaches for cancer therapy. Copyright © Experimental Oncology, 2017.EnglishAll Open Access; Green Open AccessAdipose TissueApoptosisBiomarkersCell DifferentiationCell Line, TumorCells, CulturedCoculture TechniquesCytotoxicity, ImmunologicGene ExpressionHumansKiller Cells, NaturalMesenchymal Stromal CellsMicroRNAsNeoplasmsTransfectionmicroRNAmicroRNA 150natural killer cell receptor NKG2Dunclassified drugbiological markermicroRNAMIRN150 microRNA, humanadipose tissue celladipose tissue derived mesenchymal stem cellantineoplastic activityapoptosisArticleCD16 geneCD56 genecell differentiationcoculturecontrolled studygene expression regulationgenetic transfectionGKMB genehumanhuman cellimmunohistochemistryimmunoreactivityKIR2DL2 genemesenchymal stem cellnatural killer cellNK cell cultureNKG2D geneNKp46 geneoncogenePANC-1 cell linereverse transcription polymerase chain reactionstem cell culturetumor suppressor geneTUNEL assayupregulationadipose tissuecell culturecell differentiationcytologycytotoxicitygene expressiongeneticsimmunologymesenchymal stroma cellmetabolismnatural killer cellneoplasmpathologytumor cell lineArticle10.31768/2312-8852.2017.39(3):212-218