Tokusoglu Ö.Bozoglu F.2024-07-222024-07-22201011201770http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18449Mycotoxin citrinin (CIT) and ochratoxin A (OTA) were simultaneously identified using immunoaffinity column-high performance liquid chromatography with fluorescence detection (IAC-HPLCFD) (Ex.333 nm; Em:495 nm) after an optimized extraction procedure. Both mycotoxins were eluted on a C18 RP support (250 × 4.6 mm I.D., ODS2, 5 μm particles) using an isocratic eluent consisting of acetonitrile/water/formic acid (60/38/2, v/v/v), acidified to pH 2.5 and pumped at a flow rate of 1.0 mL min-1. The four categories of citrinin levels [0-0.55; 1.56-2.0; 0.66-2.64; 5.76- 14.55 μg kg-1 of CIT ] and three categories of ochratoxin levels [0-< 0.1; 0.1-0.25; 0.30- 0.46 μg kg-1 of OTA] were found in 88 groups of olive samples. Recovery studies [y= 21416x-7919.4 (R2=0.9998) for citrinin and y= 0.0001x + 0.0074 (R2=0.9999) for ochratoxin A] were performed and the mean analytical recoveries detected in CIT and OTA in table olives ranged from 92.65-96.83% and 88.92-95.58%, respectively. Limit of detection (LOD) was equivalent to 0.05 μg/kg for both CIT and OTA. With the proposed method, CIT and OTA were both quickly determined in table olives and could be used to detect of mycotoxinic risks in a HACCP quality system of olive and olive-based food products. © 2010.EnglishOleaceaeArticle