Işisaǧ A.Neşe N.Ermete M.Lekili M.Ayhan S.Kandiloǧlu A.R.2024-07-222024-07-22200308846812http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/20157OBJECTIVE: To assess the immunoreactivity of 5 proteins related to basement membrane (BM) and extracellular matrix in order to investigate whether any of them correlates with differentiation of prostatic adenocarcinoma (PAc). Two of these markers are collagen type IV (Col IV), the collagenous component of basement membrane, and fibronectin (Fn), an adhesion protein in extracellular matrix. Others are matrix metalloproteinase-9 (MMP-9), a type IV collagenase, tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), which has a high affinity for MMP-9, and 67-kd laminin receptor (67LR), which belongs to the non-integrin laminin binding receptor family. STUDY DESIGN: Forty-three PAc cases with Gleason scores ranging between 5 and 10 and 10 benign prostatic hyperplasia (BPH) cases, the control group, were included in the study. Formalin-fixed and paraffin-embedded tissue slides from each case were immunostained with the avidin-biotin-peroxidase method. Immunoreactivity was determined by means of a scoring system similar to the Gleason scoring system. RESULTS: Overexpression of Col IV, Fn, 67LR and MMP-9 was detected in PAc as compared with BPH, whereas no difference was determined in TIMP-1 expression. Among these, only 67LR correlated statistically with Gleason score. CONCLUSION: Expression of 67LR in tumor cells was significantly increased in parallel to tumor grade. This may be useful in microscopic evaluation of PAc.EnglishAdenocarcinomaAgedAged, 80 and overBasement MembraneCase-Control StudiesCollagen Type IVExtracellular Matrix ProteinsFibrinogenGene Expression Regulation, NeoplasticHumansImmunohistochemistryMaleMatrix Metalloproteinase 9Middle AgedNeoplasm ProteinsProstatic HyperplasiaProstatic NeoplasmsReceptors, LamininSeverity of Illness IndexTissue Inhibitor of Metalloproteinase-1avidinbiotincollagen type 4fibronectingelatinase Blaminin receptorperoxidasetissue inhibitor of metalloproteinase 1adultagedarticlebasement membraneclinical articlecontrolled studyextracellular matrixgene overexpressiongleason scorehumanhuman cellhuman tissueimmunohistochemistryimmunoreactivitypriority journalprostate adenomaprostate cancerprostate hypertrophyprotein expressionscoring systemstatistical analysistissue sliceArticle