Özgül Özdemir R.B.Özdemir A.T.Kırmaz C.Eker Sarıboyacı A.Karaöz E.Erman G.Vatansever H.S.Mete Gökmen N.2024-07-222024-07-22202110434666http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13411Mesenchymal stem cells (MSCs) are powerful immunomodulatory cells. The effects of the aging on these abilities of MSCs have not been adequately clarified. In this study, alterations in immunomodulatory abilities of MSCs caused by aging were investigated. For this, dental pulp (DP) MSCs and peripheral blood mononuclear cells (PBMCs) of elderly and young donors were co-cultured age-matched and cross. We detected that the effects of DP-MSCs on Th1 and Th2 cells and their specific cytokines IFN-γ and IL-4 are not affected by aging. However, we observed that young and elderly DP-MSCs have different effects on Th17 and Treg cells. Th17 frequencies of young and elderly PBMCs were significantly increased only by young DP-MSCs, in contrast, Treg frequencies were significantly increased by elderly DP-MSCs. IL-6, IL-17a and HGF levels of both young and elderly PBMCs showed a significant increase only by young DP-MSCs, but TGF-β levels were significantly increased only by elderly DP-MSCs. The oral cavity is home to a rich microflora. The interactions of dental tissues with this microflora can lead them to acquire different epigenetic modifications. Aging can affect the microflora composition of the oral cavity and change this process in different directions. According to our findings, DP-MSCs are effective cells in the regulation of CD4+ T cells, and their effects on Th1 and Th2 cells were not affected by aging. However, pleiotropic molecules IL-6 and HGF expressions, which are important in dental and bone tissue regeneration, decreased significantly in elderly DP-MSCs. This situation may have indirectly made a difference in the modulation effects of young and elderly DP-MSCs on the Th17 and Treg cells. © 2020 Elsevier LtdEnglishAdipocytesAdultAge FactorsAgedAgingCD4-Positive T-LymphocytesCell DifferentiationCoculture TechniquesDental PulpFemaleHumansImmunomodulationLeukocytes, MononuclearMaleMesenchymal Stem CellsOsteogenesisT-Lymphocyte SubsetsT-Lymphocytes, RegulatoryTh1 CellsTh17 CellsTh2 CellsTime FactorsYoung Adultinterleukin 17interleukin 6scatter factortransforming growth factor betaadultagedagingArticleCD4+ T lymphocytecoculturecontrolled studycytokine productionDNA modificationfemalehumanhuman cellhuman experimenthuman tissueimmunomodulationlymphocyte proliferationmalemesenchymal stem cellnormal humanperipheral blood mononuclear cellpleiotropypriority journalprotein functionregulatory T lymphocyteT lymphocyte subpopulationTh1 cellTh17 cellTh2 celltissue regenerationtooth pulpadipocyteageagingbone developmentCD4+ T lymphocytecell differentiationcytologyimmunomodulationmesenchymal stem cellmetabolismmononuclear cellT lymphocyte subpopulationtime factortooth pulpyoung adultArticle10.1016/j.cyto.2020.155367