Ozbilgin A.Durmuskahya C.Kayalar H.Ertabaklar H.Gunduz C.Ural I.O.Zeyrek F.Kurt O.Cavus I.Balcıoglu C.Toz S.O.Ozbel Y.2024-07-222024-07-22201415965996http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16619Purpose: To determine the in vitro and in vivo anti-leishmanial activities of extracts obtained from Centaurea calolepis, Phlomis lycia, Eryngium thorifolium, Origanum sipyleum and Galium incanum ssp. centrale.; Methods: To estimate the cytotoxicity of plant extracts, WST-1 assay was used. Parasite inhibition in the presence of plant extracts (25-500 μg/ml) in comparision with control group and reference group (glucantime, 25 μg/ml) at 12-72 h were determined in vitro on L. tropica promastigotes. The in vivo leishmanicidal activity of the extracts was evaluated against L. tropica-infected mice with glucantime as reference drug.; Results: The chloroform extract of Galium incanum ssp. centrale showed the highest cytotoxicity with IC50 value of 0.0316 ± 0.005 μg/ml. In vitro parasite inhibition by the plant extracts ranged between 16.7 ± 0.01 % and 100 ± 0.00 % at 25 μg/ml concentration. The methanol extract of Eryngium thorifolium possessed the highest activity on promastigotes of L. tropica with 100 % inhibition at 25 μg/ml. The water and chloroform extracts of C. calolepis and water and methanol extracts of E. thorifolium at a dose of 100 mg/kg reduced parasitaemia in L. tropica infected mice.; Conclusion: Parasite viability results suggest that the methanol extract of Eryngium thorifolium, regarded as non-cytotoxic, is a promising candidate drug for treating L. tropica infection. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.EnglishAll Open Access; Gold Open Access; Green Open Accesscentaurea calolepis extracteryngium thorifolium extractgalium incanum extractmeglumine antimonateoriganum sipyleum extractphlomis lycia extractplant extractunclassified druganimal experimentanimal modelanimal tissueantileishmanial activityantiprotozoal activityArticlecontrolled studycytotoxicityfemaleIC50leishmaniasismousenonhumanpromastigoteskin leishmaniasisskull injuryWST-1 assayArticle10.4314/tjpr.v13i12.15