Günşar C.Vatansever H.S.Arslan O.A.Şencan A.Müftüoǧlu S.Özbilgin K.Kaymaz F.Mir E.2024-07-222024-07-22200400223468http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19921Background/purpose The maturity of neomucosa growing on a serosal surface for the treatment of short bowel syndrome still is questionable. The aim of this study was to evaluate the intestinal neomucosa to assess its histologic maturity. Methods A 6-cm-long isolated ileal segment (IS) was prepared in 8 Wistar albino-type rats. The IS was divided from the antimesenteric side, and 2 intestinal tubes were established, which shared a common wall and a common pedicle. After ileal biopsy sampling for the control group (CG), the IS was fashioned into a mucous fistula. Eight weeks later, all the rats were killed, and the ISs were investigated for neomucosal growth. Sections were prepared with periodic acid shift (PAS) and H & E staining for light microscopy. They also were evaluated by transmission electron microscopy. The microscopic morphology of the 2 groups was evaluated. Immunohistochemical staining was performed to show the expression of the tissue β1, α3 and α2β1 integrin subunits of both the neomucosa (NS) and control group (CG) segments. Results Sections of the NS showed a well-arranged columnar epithelial cell layer with goblet cells that were generally located superficially and with a complete basement membrane. Under the electron microscope, the sections from the NS group showed an epithelial cell layer with proper microvilli of the same height, although they were shorter than those of the CG, and tight intercellular junctions between the epithelial cells. Significant differences between the NS and CG groups were found in the measurements of villus width at base, microvillus surface, and microvillus height. The lamina propria consisted of rich collagen fibers and active fibroblasts in the NS group. In the immunohistochemical staining, although β1 integrine showed a dense distribution (+++) in the lamina propria, particularly localizing at the depth of the tunica mucosa layer, α3 integrin was observed to have a less dense immunoreactivity (++) in both groups. The expression of α2β1 integrin showed slight and dispersed (+) staining. Conclusions The NS showed histologic maturity and ultimate structural similarity with the native small bowel mucosa, which provides strong indirect evidence for the proper functioning of the neomucosa. © 2004 Elsevier Inc. All rights reserved.EnglishAnastomosis, SurgicalAnimalsAntigens, CD29BiopsyEpithelial CellsIleumIntegrin alpha2beta1Integrin alpha3Intestinal MucosaMicroscopy, ElectronMicrovilliRatsRats, WistarShort Bowel SyndromeStaining and LabelingSurgically-Created StructuresWound Healingalpha3 integrinbeta1 integrincollagen fibereosinhematoxylinprotein subunitvery late activation antigen 2albinismanimal experimentanimal tissuearticlebasement membranecell activationcell junctioncell surfacecellular distributioncolumnar epitheliumcontrolled studydispersionevaluationfibroblastgoblet cellheighthistologyileumimmunohistochemistryimmunoreactivityintestine fistulalamina propriamesenterymicromorphologymicrovillusnonhumanobservationperiodic acid Schiff stainpriority journalprotein expressionprotein localizationprotein structureratsmall intestine mucosatissue growthtransmission electron microscopyultrastructureArticle10.1016/j.jpedsurg.2004.05.008