Pala H.G.Pala E.E.Artunc Ulkumen B.Aktug H.Yavasoglu A.Korkmaz H.A.Erbas O.2024-07-222024-07-22201403787346http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16903Aims: To evaluate the effect of granulocyte colony-stimulating factor (G-CSF) on the endometrium and ovaries in an experimental diabetes mellitus (DM) rat model. Methods: A total of 18 female Sprague-Dawley albino mature rats (8 weeks, 200-220 g) were used in this study. Diabetes was induced by intraperitoneal (i.p.) injection of streptozocin randomly in 12 rats. No drug was administered to the remainder of the rats (control group, group 1, n = 6). The other 12 rats were randomly divided into 2 groups; 1 ml/kg i.p. saline was given as vehicle to group 2 (diabetic nontreated control group, n = 6) and 100 μg/kg/day of i.p. G-CSF was given to group 3 (G-CSF-treated group, n = 6) for 4 weeks. After 4 weeks, blood samples were collected and hysterectomy with bilateral oophorectomy was performed for histopathological examination. Results: The mean endometrial gland degeneration and stromal fibrosis scores were significantly higher in group 2 compared with groups 1 and 3. Ovarian follicle degeneration, stromal degeneration and stromal fibrosis scores were significantly higher in group 2 compared with groups 1 and 3. Plasma TGF-β and malondialdehyde levels were significantly lower in groups 1 and 3 compared with group 2. Antimüllerian hormone levels were significantly lower in group 2 compared with groups 1 and 3. Conclusion: Glucose toxicity occurred severely in the ovaries and endometrium of the DM rats. After G-CSF treatment, ovarian and endometrial injury and fibrosis scores decreased significantly. The effects of G-CSF in rat models give hope to improved treatment of human DM complications such as premature ovarian failure and endometrial dysfunction. © 2014 S. Karger AG, Basel.EnglishAnimalsDiabetes Mellitus, ExperimentalEndometriumFemaleGranulocyte Colony-Stimulating FactorMalondialdehydeOvarian DiseasesOvaryPrimary Ovarian InsufficiencyRatsRats, Sprague-DawleyTransforming Growth Factor betaUterine Diseasesglucosegranulocyte colony stimulating factormalonaldehydeMuellerian inhibiting factorrecombinant granulocyte colony stimulating factorstreptozocinthiobarbituric acid reactive substancetransforming growth factor betatrichloroacetic acidfilgrastimsodium chloridegranulocyte colony stimulating factormalonaldehydetransforming growth factor betaanimal experimentanimal modelanimal tissuearticlecontrolled studydegenerationdrug effectendometrial diseaseendometrial gland degenerationendometrial stromal fibrosisfemalefibrosisglucose blood levelglucotoxicityhistopathologyhysterectomyinjury severitylipid peroxidationnonhumanovarian follicle degenerationovarian stromal fibrosisovariectomyovary diseaseovary follicleoxidative stresspriority journalprotein blood levelratstreptozotocin-induced diabetes mellitusstromal degenerationArticleblood samplingendometriumexperimental diabetes mellitusovarytissue degenerationanimalbloodcomplicationendometriumexperimental diabetes mellitusOvarian DiseasesovarypathologyPrimary Ovarian InsufficiencySprague Dawley ratUterine DiseasesArticle10.1159/000363239