Özdemir A.T.Özgül Özdemir R.B.Kırmaz C.Sarıboyacı A.E.Ünal Halbutoğlları Z.S.Özel C.Karaöz E.2024-07-222024-07-22201600088749http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15623Mesenchymal stem cells (MSCs) have strong immunomodulatory properties, however these properties may show some differences according to the tissue type of their isolate. In this study we investigated the paracrine interactions between human DP derived MSCs (hDP-MSCs) and the CD4+ T helper cell subsets to establish their immunomodulatory mechanisms. We found that the CD4+-Tbet+ (Th1) and CD4+-Gata3+ (Th2) cells were suppressed by the hDP-MSCs, but the CD4+-Stat3+ (Th17) and CD4+-CD25+-FoxP3+ (Treg) cells were stimulated. The expressions of T cell specific cytokines interferon gamma (IFN-g), interleukin (IL)-4 and IL-17a decreased, but IL-10 and transforming growth factor beta-1 (TGF-b1) increased with the hDP-MSCs. The expressions of indoleamine-pyrrole 2,3-dioxygenase (IDO), prostaglandin E2 (PGE2), soluble human leukocyte antigen G (sHLA-G) derived from hDP-MSCs slightly increased, but hepatocyte growth factor (HGF) significantly increased in the co-culture groups. According to our findings, the hDP-MSCs can suppress the Th1 and Th2 subsets but stimulate the Th17 and Treg subsets. The Stat3 expression of Th17 cells may have been stimulated by the HGF, and thus the pro-inflammatory Th17 cells may have altered into the immunosuppressive regulatory Th17 cells. Further prospective studies are needed to confirm our findings. © 2016 Elsevier Inc.EnglishCell DifferentiationCells, CulturedDental PulpDinoprostoneForkhead Transcription FactorsGATA3 Transcription FactorHepatocyte Growth FactorHumansImmunomodulationIndoleamine-Pyrrole 2,3,-DioxygenaseMesenchymal Stromal CellsParacrine CommunicationSTAT3 Transcription FactorT-Box Domain ProteinsT-Lymphocytes, RegulatoryTh1 CellsTh17 CellsTh2 CellsCD4 antigengamma interferonHLA G antigenindoleamine 2,3 dioxygenaseinterleukin 10interleukin 17interleukin 2 receptor alphainterleukin 4prostaglandin E2scatter factor receptortranscription factor FOXP3transcription factor GATA 3transforming growth factor beta1forkhead transcription factorFOXP3 protein, humanGATA3 protein, humanHGF protein, humanindoleamine 2,3 dioxygenaseprostaglandin E2scatter factorSTAT3 proteinT box transcription factorT-box transcription factor TBX21transcription factor GATA 3adaptive immunityadultArticleblood samplingCD4+ T lymphocytecell interactioncell isolationcell stimulationcoculturecontrolled studyeffector cellfemaleflow cytometryhumanhuman cellimmunomodulationlymphocyte differentiationlymphocyte proliferationmesenchymal stem cellnormal humanpriority journalprotein expressionregulatory T lymphocyteT lymphocyte subpopulationTh1 cellTh17 cellTh2 celltooth pulpyoung adultcell culturecell differentiationimmunologyimmunomodulationmesenchymal stroma cellmetabolismparacrine signalingpathologyphysiologyArticle10.1016/j.cellimm.2016.08.008