Işın AKYARCengiz ÇAVUŞOĞLUMeltem AYASSüheyla SÜRÜCÜOĞLUZEYNEP ARZU İLKİDeniz Ece KAYAYeşim BEŞLİ2024-07-242024-07-2420181300-0144http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/25384Background/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way ofidentifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permitsearly, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy ofphenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) comparedto standard sequence analysis.Materials and methods: A total of 155 Mycobacterium clinical and external quality control isolates, comprising nontuberculousmycobacteria (NTM) (n = 95) and the Mycobacterium tuberculosis complex (MTC) (n = 60), were included in the study.Results: Identification by MBT ML4.0 was correctly performed in 100% of MTC and in 91% of NTM isolates. All of the MTC isolateswere correctly differentiated from NTM isolates.Conclusion: Based on our results, MBT ML4.0 may be used reliably to identify both NTM and MTC.eng[Fen > Tıp > Cerrahi]Araştırma Makalesi10.3906/sag-1801-198