Atmaca H.Çamlı Pulat Ç.Ilhan S.2024-07-222024-07-22202226768615http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12531In this study, silver nanoparticles were synthesized using Alpinia officinarum rhizome extract via an eco-friendly green synthesis method. The silver nanoparticles (AO-AgNPs) were characterized by UV–Vis spectrometry, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and dynamic light scattering. Further, the cytotoxic and apoptotic effects of AO-AgNPs were investigated in human cancer cells with different tissue origins via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and flow cytometric analyses, respectively. The expression levels of anti-apoptotic Bcl-2 protein were evaluated via a real-time polymerase chain reaction. The synthesized AO-AgNPs induced a significant cytotoxic effect in all tested cancer cells but not in normal cells. AO-AgNPs induced the percentage of apoptotic cells and reduced the levels of anti-apoptotic Bcl-2 mRNA levels in cancer cells. These results demonstrate the potential application of AO-AgNPs in cancer treatment. © 2022, Akadémiai Kiadó Zrt.EnglishAlpiniaAntineoplastic AgentsApoptosisBromidesHumansMetal NanoparticlesNeoplasmsPlant ExtractsRhizomeRNA, MessengerSilver3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromideAlpinia officinarum extractantineoplastic agentmessenger RNAplant extractprotein bcl 2silver nanoparticleunclassified drugantineoplastic agentbromidemessenger RNAmetal nanoparticleplant extractsilverAlpinia officinarumapoptosisapoptotic cell percentageArticlecontrolled studydown regulationdrug cytotoxicitydrug synthesisDU145 cell lineenergy dispersive X ray spectroscopyflow cytometryH69AR cell linehumanhuman cellMCF-7 cell linemRNA expression levelMTT assayphoton correlation spectroscopyprotein expressionreal time polymerase chain reactionrhizomescanning electron microscopyultraviolet visible spectroscopyAlpiniaapoptosismetabolismneoplasmrhizomeArticle10.1007/s42977-022-00132-5