Şiyve S.Ulusoy O.Karakuş O.Z.Murat N.Uslu M.E.Ateş O.Hakgüder G.Olguner M.Akgür F.M.2024-07-222024-07-22201700223468http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15471Objective Intraamniotic meconium has been responsible for intestinal damage in gastroschisis and meconium-dependent intestinal ischemia has been proposed to induce additional intestinal damage in gastroschisis. This study is aimed to determine the effects of lipid and water-soluble meconium subfractions on the contractility of the superior mesenteric artery (SMA). Materials and methods The study was conducted on 18-day fertilized chick embryos (Gallus Domesticus). Meconium is fractioned into water and lipid-soluble components. Only one SMA tissue was prepared from each embryo and suspended in the organ bath. Isometric contraction responses (ICR) were created in SMA tissues by one hour of incubation in Krebs–Henseleit solution for each group. Groups consisted of control, meconium, water-soluble meconium subfraction and lipid-soluble meconium subfraction. ICR of the SMA specimens were evaluated with a transducer-amplifier system on a computer. The data were expressed (mean ± 1SD) as milliNewton (mN). Results The ICR of the meconium, water-soluble meconium subfraction and lipid-soluble meconium subfraction groups were significantly high when compared to the control group (p < 0.01). The meconium and water-soluble meconium subfraction created more contraction response than the lipid-soluble meconium subfraction (p < 0.01). The ICR of the meconium group was not different from the ICR of the water-soluble meconium subfraction group (p > 0.05). Conclusion Water-soluble meconium subfraction has a profound vasoconstrictor effect on the SMA compared to the lipid-soluble meconium subfraction. © 2016 Elsevier Inc.EnglishAnimalsChick EmbryoGastroschisisGlucoseIntestinal DiseasesIntestinesLipidsMeconiumMesenteric Artery, SuperiorTromethamineVasoconstrictionWaterglucoseKrebs-Henseleit solutionlipidtrometamolwateranimal tissueartery constrictionArticleblood vessel parameterschick embryochickencomparative studycontrolled studyembryointestine injuryintestine ischemiaisometric contraction responselipid soluble meconiummeconiumnewbornnonhumanpriority journalsmall intestinesuperior mesenteric arterywater soluble meconiumanimalchemistrycomplicationgastroschisisIntestinal Diseasesintestinemeconiumpathophysiologysuperior mesenteric arteryvasoconstrictionArticle10.1016/j.jpedsurg.2016.09.066