Çiftdoǧan D.Y.Coşkun Ş.Ulman C.Tkz H.2024-07-222024-07-22200909546928http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18669Atherosclerosis, the major cause of coronary artery disease (CAD), has a very long asymptomatic development phase, which begins in childhood. In this study, we describe the Factor V G1691A, Factor V H1299R and prothrombin G20210A gene polymorphisms in children with a family history of premature CAD. Evidence of these polymorphisms in these children may predict the probability of having atherosclerosis in the future. Our study included a total of 140 children, 72 males and 68 females between the ages of 4.9 and 15.7 years. Among these children, 73 had a parental history of premature CAD and the remaining 67 belonged to our control group. The participants were screened for the mutations Factor V G1691A, Factor V H1299R and prothrombin G20210A by polymerase chain reaction amplified DNA products with specific oligonucleotide probes. Our results suggested that frequencies of the mutated allele of Factor V G1691A and prothrombin G20210A are higher in children with a parental history of premature CAD. In conclusion, Factor V G1691A and prothrombin G20210A polymorphisms which were detected in higher frequencies in children with a parental history of premature CAD may indicate a risk for developing atherosclerosis and might be useful in screening for CAD in children; however, large population-based research is necessary to investigate further genetic risk assessment for CAD. © 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins.EnglishAdolescentAge of OnsetAtherosclerosisCase-Control StudiesChildChild, PreschoolCoronary Artery DiseaseFactor VFemaleGene FrequencyGenetic Association StudiesGenetic Predisposition to DiseaseHumansMaleMutationPedigreePolymorphism, GeneticProthrombinRisk AssessmentRisk Factorsalaninearginineblood clotting factor 5DNAglycinehistidineprothrombinadolescentarticleatherosclerosischildcontrolled studycoronary artery diseasefamily historyfemalegene amplificationgene frequencygenetic polymorphismgenetic riskgenetic screeninggenotypehumanmajor clinical studymalemutational analysisoligonucleotide probeparentpolymerase chain reactionpredictionpreschool childpriority journalprobabilityrisk assessmentschool childArticle10.1097/MCA.0b013e32832bdb8c