Göçmen C.Göktürk H.S.Ertuǧ P.U.Önder S.Dikmen A.Baysal F.2024-07-222024-07-22200000142999http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/20542The effects of neocuproine and bathocuproine, Cu(I) and Cu(II) chelators, respectively, were studied on relaxations in response to electrical field stimulation, acetylcholine, S-nitrosoglutathione, acidified sodium nitrite and sodium nitroprusside in the mouse corpus cavernosum precontracted with phenylephrine. Neocuproine significantly inhibited relaxations induced by electrical field stimulation, acetylcholine and S- nitrosoglutathione, but not by acidified sodium nitrite and sodium nitroprusside. The pre-prepared neocuproine-Cu(I) complex was ineffective on the responses. The discrepancy between the shape of relaxations in response to electrical field stimulation or to acetylcholine and S-nitrosoglutathione was abolished by adding CuCl2 into the bathing medium. The copper action was blocked by neocuproine but not by bathocuproine. However, the pre-prepared bathocuproine-Cu(II) complex did not accelerate the relaxations affected by CuCl2. These findings suggest that a Cu(I)-dependent mechanism may play a role in the relaxation induced by the endogenous relaxant factor as well as by S-nitrosoglutathione in mouse cavernosal tissue. (C) 2000 Elsevier Science B.V.EnglishAcetylcholineAnimalChelating AgentsCopperDose-Response Relationship, DrugElectric StimulationGlutathioneMaleMiceNitric OxideNitroprussideNitroso CompoundsPenisPhenanthrolinesPhenylephrineS-NitrosoglutathioneSupport, Non-U.S. Gov't2,9 dimethyl 1,10 phenanthrolineacetylcholinechelating agentcopper chloridecopper ionnitroprusside sodiumphenylephrines nitrosoglutathionesodium nitriteanimal tissuearticlechelationcontrolled studycorpus cavernosumelectrostimulationmalemousenonhumanpriority journalsignal transductionsmooth muscle relaxationArticle10.1016/S0014-2999(00)00657-9