Afacan B.Keleş Yücel Z.P.Paşali Ç.Atmaca İlhan H.Köse T.Emingil G.2024-07-222024-07-22202000223492http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13866Background: Hypoxia-inducible angiogenic pathway involving hypoxia inducible factor-1 alpha (HIF-1α), vascular endothelial growth factor (VEGF) and tumor necrosis factor-alpha (TNF-α) may regulate several biological processes related to inflammation. The present study aimed to assess the effect of non-surgical periodontal treatment on gingival crevicular fluid (GCF) HIF-1α, VEGF, and TNF-α levels in generalized aggressive periodontitis (G-AgP). Methods: Twenty G-AgP patients and 20 periodontally healthy individuals were included. G-AgP patients received scaling and root planning (SRP), per quadrant at a 1-week-interval, performed with ultrasonic and periodontal hand instruments. GCF samples were collected and clinical periodontal parameters including probing depth, clinical attachment level, gingival index and plaque index were recorded at baseline, 1 and 3 months after treatment. Biomarker levels in GCF were analyzed by ELISA. Results: At baseline all clinical parameters and GCF HIF-1α, VEGF, and TNF-α levels were significantly higher in G-AgP patients compared to healthy control (P < 0.05). All clinical parameters improved over the 3-month-period in G-AgP patients (P < 0.05). GCF HIF-1α levels in G-AgP reduced at 1 and 3 months post-treatment, however, this did not reach to statistical significance (P > 0.05). GCF VEGF and TNF-α levels remained unchanged throughout the study period (P > 0.05). Conclusions: Within the limitations of the present study, although HIF-1α seems to possess a potential diagnostic value for G-AgP, it might not be a proper predictor of clinically favorable treatment outcome. SRP plus different adjunctive therapies could provide better information about the prognostic role of hypoxia-inducible angiogenic pathway in G-AgP. © 2020 The Authors. Journal of Periodontology published by Wiley Periodicals, Inc. on behalf of American Academy of PeriodontologyEnglishAll Open Access; Hybrid Gold Open AccessAggressive PeriodontitisDental ScalingGingival Crevicular FluidHumansHypoxiaTumor Necrosis Factor-alphaVascular Endothelial Growth Factor Ahypoxia inducible factor 1alphatumor necrosis factorvasculotropintumor necrosis factorvasculotropin Aadultanalytical parametersArticlebleeding on probingclinical articleclinical attachment levelcontrolled studydental disease assessmentenzyme linked immunosorbent assayfemalefirst molargeneralized aggressive periodontitisgingival indexgingivitishumanhypoxiamalemiddle agedosteolysisperiodontal diseaseperiodontitisprobing depthroot lengthroot planingsignal transductionaggressive periodontitischemistrydental scalinggingivitishypoxiaArticle10.1002/JPER.19-0521