Alioglu E.Turk U.Cam S.Abbasaliyev A.Tengiz I.Ercan E.2024-07-222024-07-2220090828282Xhttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18909Background: Single nucleotide polymorphisms in the 5,10-methyl-enetetrahydrofolate reductase (MTHFR), vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), monocyte chemoattractant protein-1 (MCP-1) and apolipoprotein E (ApoE) genes appear to be a genetic risk factor for atherosclerosis. Common carotid intima-media thickness (cIMT) provides information on the severity of atherosclerosis. Objective: To investigate the relationship between cIMT and gene polymorphisms associated with atherosclerosis in Turkish patients with coronary artery disease (CAD). Methods: Sixty-two patients with angiographically diagnosed stable CAD were divided into two groups according to their cIMT values (group 1: n=35, cIMT of 1 mm or greater; group 2: n=27, cIMT of less than 1 mm). MTHFR 677 C/T, VEGF -460 C/T, eNOS 894 G/T, MCP-1 -2518 A/G and ApoE (E2, E3 and E4) gene polymorphisms (where A is adenine, C is cytosine, G is guanine and T is thymine) were analyzed by polymerase chain reaction and restriction fragment length polymorphism. Evaluations of cardiovascular risk factors and coronary atherosclerotic lesions were performed in all patients. Serum homocysteine and high-sensitivity C-reactive protein were measured and compared between the two groups. Results: Serum high-sensitivity C-reactive protein (P=0.04) and homocysteine (P=0.006) levels were higher in group 1 than in group 2. The ratio of multivessel CAD and previous myocardial infarction was significantly higher in group 1 than in group 2 (P=0.014). In the study population, no significant difference in cIMT was observed according to the polymorphisms studied. Only hyperhomocysteinemia (OR 1.17 [95% CI 1.01 to 1.35], P=0.033) and previous myocardial infarction (OR 3.76 [95% CI 1.10 to 12.81], P=0.034) maintained a significant correlation with cIMT on multiple logistic regression analysis. Conclusion: cIMT is increased in patients with hyperhomocysteinemia, inflammation and extended CAD. MTHFR 677 C/T, VEGF -460 C/T, eNOS 894 G/ T, MCP-1 -2518 A/G and ApoE single nucleotide polymorphisms were not associated with increased cIMT. © 2009 Pulsus Group Inc. All rights reserved.EnglishAll Open Access; Green Open Access5,10 methylenetetrahydrofolate reductase (FADH2)apolipoprotein EC reactive proteinendothelial nitric oxide synthasehomocysteinemonocyte chemotactic protein 1vasculotropinadultamino acid blood levelartery intima proliferationarticlecardiovascular riskcoronary artery atherosclerosiscoronary artery diseaseDNA polymorphismfemalegenetic associationgenotypeheart infarctionhumanmajor clinical studymalepolymerase chain reactionprotein blood levelrestriction fragment length polymorphismrisk assessmentsingle nucleotide polymorphismTurkey (republic)Article10.1016/S0828-282X(09)70022-4