Ocak F.Avsever L.Turkyilmaz M.K.Turkyilmaz S.2024-07-222024-07-22202303349152http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12180Mycoplasma mastitis is a highly contagious disease of dairy cattle that generally does not respond to treatment, adversely affect milk yield as well as animal health, causing significant economic losses. Therefore, rapid and reliable identification of this pathogen is required to develop control strategies on farms. One of the most important mycoplasma agents causing mastitis is Mycoplasma bovis, an invasive agent. In this study, it was aimed to identify Mycoplasma spp. and M. bovis DNA by PCR, which are important mastitis pathogens but often neglected, in cow milk with mastitis. For this purpose, 312 milk samples with mastitis, 84 with clinical and 228 with subclinical mastitis, were investigated from 17 farms. DNA extraction from milk samples was carried out using the phenol chloroform method. Identification at the species and genus level were performed by polymerase chain reaction (PCR). In PCR, primers targeting 16S rDNA for Mycoplasma spp. and uvrC gene target regions for M. bovis were used. Mycoplasma spp. and M. bovis DNA were detected in milk samples at a rate of 19.6% (61/312) and 13.8% (43/312) respectively from 11 farms. The rate of M. bovis among all mycoplasmas was determined as 70.5% (43/61). Isolation of mycoplasmas, which are the causative agents of mycoplasma mastitis, by classical conventional methods tends to be long and laborious. Where mycoplasmal mastitis is suspected, bacterial DNA detection by PCR may be an ideal way to make a diagnosis in a short time. However, in order to develop accurate treatment strategies, it would be beneficial to examine all mycoplasma agents along with not overlooking other pathogens that could lead to mastitis. © 2023, Israel Veterinary Medical Association. All rights reserved.EnglishArticle