Erkan S.O.Tuhanioğlu B.Gürgen S.G.Özdaş T.Taştekin B.Pelit A.Görgülü O.2024-07-222024-07-2220190023852Xhttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14693Objectives/Hypothesis: The aim of this study was to investigate changes in the cochlea and the potential dose-dependent effects of resveratrol (RSV) against diabetes mellitus (DM) ototoxicity. Study Design: Animal model. Methods: Twenty-four male Wistar albino rats were divided into four groups. Baseline distortion product otoacoustic emission (DPOAE) measurements were evaluated. Group I was the control group, group II was made diabetic with single-dose streptozotocin, and groups III and IV were rendered diabetic as group II and administered 10 and 20 mg RSV, respectively, intraperitoneally for 4 weeks. All animals were sacrificed after repeated DPOAE measurement. Apoptosis was investigated using caspase-3, Bax (Bcl-associated X protein), and TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) staining. Results: The DPOAE values in the diabetic group were found to be significantly lower compared with the other groups at 5,714 Hz and 8,000 Hz (P <.05). No significant difference in otoacoustic emission was detected in the comparison of the RSV doses (P >.05). The histopathologic investigation using caspase-3, Bax, and TUNEL staining showed that the mean rank of the diabetic group was significantly higher compared with the RSV10, RSV20, and control groups (DM > RSV10 > RSV20 > control) (P <.05). Conclusions: These results imply that RSV administration offered statistically significant protection for the cochleas of rats against diabetes. This protective effect improved histologically with higher doses. Level of Evidence: NA Laryngoscope, 129:E1–E6, 2019. © 2018 The American Laryngological, Rhinological and Otological Society, Inc.EnglishAnimalsApoptosisCochleaDiabetes Mellitus, ExperimentalDisease Models, AnimalDose-Response Relationship, DrugImmunohistochemistryIn Situ Nick-End LabelingMaleOtoacoustic Emissions, SpontaneousRatsRats, WistarResveratrolcaspase 3protein Baxresveratrolresveratrolanimal experimentanimal modelArticlecochleacontrolled studyCorti organdistortion product otoacoustic emissionhistopathologyimmunohistochemistrymalenonhumanototoxicitypriority journalratsignal noise ratiostreptozotocin-induced diabetes mellitusTUNEL assayanimalapoptosiscochleadisease modeldose responsedrug effectexperimental diabetes mellituspathologyspontaneous otoacoustic emissionWistar ratArticle10.1002/lary.27253