Okay S.Çetin R.Karabulut F.Dogan C.Sürücüoglu S.Kizildogan A.K.2024-07-222024-07-22201812178950http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15079Immunogenic potency of the recombinant Erp, HspR, LppX, MmaA4, and OmpA proteins from Mycobacterium tuberculosis (MTB), formulated with Montanide ISA 720 VG adjuvant, was evaluated in BALB/c mice for the first time in this study. The five vaccine formulations, adjuvant, and BCG vaccine were subcutaneously injected into mice, and the sera were collected at days 0, 15, 30, 41, and 66. The humoral and cellular immune responses against vaccine formulations were determined by measuring serum IgG and serum interferon-gamma (IFN-γ) and interleukin-12 (IL-12) levels, respectively. All formulations significantly increased IgG levels post-vaccination. The highest increase in IFN-γ level was provided by MmaA4 formulation. The Erp, HspR, and LppX formulations were as effective as BCG in enhancement of IFN-γ level. The most efficient vaccine boosting the IL-12 level was HspR formulation, especially at day 66. Erp formulation also increased the IL-12 level more than BCG at days 15 and 30. The IL-12 level boosted by MmaA4 formulation was found to be similar to that by BCG. OmpA formulation was inefficient in enhancement of cellular immune responses. This study showed that MmaA4, HspR, and Erp proteins from MTB are successful in eliciting both humoral and cellular immune responses in mice. © 2018 Akadémiai Kiadó, BudapestEnglishAll Open Access; Green Open AccessAnimalsAntibodies, BacterialBacterial Outer Membrane ProteinsBacterial ProteinsBCG VaccineCytokinesFemaleHeat-Shock ProteinsImmunity, CellularImmunity, HumoralMannitolMembrane ProteinsMethyltransferasesMiceMice, Inbred BALB CMixed Function OxygenasesMycobacterium tuberculosisOleic AcidsRepressor ProteinsTuberculosisTuberculosis VaccinesVaccinationVaccines, Syntheticbacterial vaccineBCG vaccineErp proteingamma interferonHspR proteinimmunoglobulin Gimmunological adjuvantinterleukin 12LppX proteinMmaA4 proteinouter membrane protein Arecombinant proteinrecombinant vaccineunclassified drugbacterial proteinbacterium antibodyBCG vaccinecytokineERP protein, Mycobacterium tuberculosisheat shock proteinHspR protein, bacteriamannide monooleatemannitolmembrane proteinmethyltransferasemixed function oxidasemma4 protein, Mycobacterium tuberculossisoleic acidOMPA outer membrane proteinsouter membrane proteinrecombinant vaccinerepressor proteinanimal experimentanimal modelArticlebacterial strainbacterium isolateBCG vaccinationcellular immunitycontrolled studydrug formulationfemalehumanhumoral immunityimmune responseimmunoglobulin blood levelmouseMycobacterium tuberculosisnonhumanprotein blood leveltuberculosisvaccine immunogenicityanimalBagg albino mousebloodgeneticsimmunologymicrobiologytuberculosisvaccinationArticle10.1556/030.65.2018.048