Vatansever H.S.Inan V.S.Lacin S.Koyuncu F.2024-07-222024-07-22200300651281http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/20210Signs of pre-eclampsia are considered to be caused by maternal endothelial dysfunction due to circulating factors of placental origin. Integrins are a large family of cell surface proteins that serve as receptors involved in cell-cell and cell-matrix interactions during placentation. Therefore, low expression of integrins or the lack of it may be encountered during pre-eclampsia. In the present study, we investigated the immunolocalisation of integrins αV, α3 and β1 in placentas of normal and preeclamptic women. Thirty-two placentas from pre-eclamptic (n = 14) and normotensive (n = 18) women were used. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissue specimens, using anti-αV, anti-α3 and anti-β1 antibodies and the indirect immunoperoxidase technique. A semi-quantitative grading system (HSCORE) was used to compare immunohistochemical staining intensities. Distribution patterns of αV, α3 and β1 integrins were detected in cytotrophoblasts and Hofbauer cells in normal and pre-eclamptic placentas. Immunostaining of αV and β1 integrins was slightly decreased in pre-eclamptic samples but α3 integrin immunostaining was similar in pre-eclamptic and normal placentas. Decreased immunostaining of integrins in the cytotrophoblasts may considered to be a structural basis for decreased placental perfusion in pre-eclampsia.EnglishAntigens, CD29FemaleHumansImmunohistochemistryIntegrin alpha3Integrin alphaVPlacentaPre-EclampsiaPregnancyAntibodiesEnzyme kineticsHistologyImmunologyalpha3 integrinalpha5 integrinbeta1 integrinformaldehydeintegrinmonoclonal antibodyparaffinperoxidaseunclassified drugalpha3 integrinbeta1 integrinCD51 antigenImmunostainingsadultarticlecell functionclinical articlecontrolled studycytotrophoblastendothelium cellfemalehumanhuman tissueimmunohistochemistryimmunolocalizationplacentapreeclampsiaprotein expressionprotein familyprotein functionprotein localizationscoring systemchemistrycytologyhistologyimmunologymetabolismpregnancyTissue cultureArticle10.1078/0065-1281-00708