Dincer F.Atmaca H.Akman L.Oktay L.M.Karaca B.Terek M.C.2024-07-222024-07-22202321678359http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12360Background: Obesity is associated with increased mortality among ovarian cancer and is a poor prognostic factor. There are significant links between the leptin hormone, a product of the obesity gene, and the development of ovarian cancer. Leptin is a vital hormone-like cytokine secreted from adipose tissue and is mainly involved in the maintenance of energy homeostasis. It regulates several intracellular signaling pathways and also interacts with various hormones and energy regulators. It acts as a growth factor by stimulating cell proliferation and differentiation and in this way contributes to cancer cell development. The aim of the study was to investigate the effects of leptin on human ovarian cancer cells. Methods: In this study, the effects of increasing the concentration of leptin were investigated on the cell viability of OVCAR-3 and MDAH-2774 ovarian cancer lines by MTT assay. Moreover, to elucidate the molecular mechanisms of leptin in ovarian cancer cells, changes in the expression levels of 80 cytokines were evaluated after leptin treatment via a human cytokine antibody array. Results: Leptin increases the proliferation of both ovarian cancer cell lines. IL-1 level was increased in OVCAR-3 cells and TGF-β level was increased in MDAH-2774 cells after leptin treatment. A decrease in IL-2, MCP-2/CCL8 and MCP-3/CCL7 levels was detected in both ovarian cancer cell lines with leptin administration. An increase in IL-3 and IL-10 expressions, insulin-like growth factor binding proteins (IGFBP) IGFBP-1, IGFBP-2 and IGFBP-3 levels were detected in both ovarian cancer cell lines with leptin administration. In conclusion; leptin has a proliferative effect on human ovarian cancer cell lines and affects different cytokines in different types of ovarian cancer cells. Copyright 2023 Dincer et al.EnglishAll Open Access; Gold Open Access; Green Open Accesscytokineepidermal growth factorfibroblast growth factor 6interleukin 1interleukin 10interleukin 12interleukin 13interleukin 16interleukin 2interleukin 3interleukin 5interleukin 8leptinmonocyte chemotactic protein 2monocyte chemotactic protein 3placental growth factorplatelet derived growth factor BBsomatomedin binding protein 1somatomedin binding protein 2somatomedin binding protein 3transforming growth factor betaArticlecell proliferationcell viabilitycell viability assaychemoluminescencecontrolled studyfemalehumanhuman cellMDAH 2774 cell lineMTT assayobesityovarian cancer cell lineovary cancerOVCAR-3 cell lineXTT assayArticle10.7717/peerj.15246