Salmanoglu D.S.Gurpinar T.Vural K.Ekerbicer N.Dariverenli E.Var A.2024-07-222024-07-22201622132317http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15751Vascular dysfunction is thought to play a major role in the development of diabetic cardiovascular disease. The roles of endothelial dysfunction, oxidative stress, and dyslipidemia will be considered. Melatonin as well as L-carnitine were shown to possess strong antioxidant properties. Diabetes induced with high fat diet (for 8 weeks) and multipl low doses intraperitoneal injection of STZ (twice, 30. mg/kg/d i.p). The diabetic animals were randomly assigned to one of the experimental groups as follows: Control group (C), high fat diet (HFD), STZ-induced diabetic group (HFD+STZ) , HFD+STZ diabetic group received melatonin (10 mg/kg/d i.p), HFD+STZ diabetic group received L-carnitine (0.6 g/kg/d i.p), and HFD+STZ diabetic group received glibenclamide (5 mg/kg/d, oral). The serum fasting blood glucose, insulin, total cholesterol, HDL- cholesterol, LDL-cholesterol, triglyceride and malondialdehyde (MDA) levels were tested. Acetylcholine induced endothelium-dependent relaxation and sodium nitroprusside induced endothelium-independent relaxation were measured in aortas for estimating endothelial function. Also, glutathione peroxidase (GP x), superoxide dismutase (SOD) and nitric oxide (NO) levels activities were determined in rat liver. According to our results melatonin and L-carnitine treatment decreased fasting blood glucose, total cholesterol, and LDL levels. MDA levels significantly decreased with the melatonin treatment whereas SOD levels were not significantly changed between the groups. The results suggest that especially melatonin restores the vascular responses and endothelial dysfunction in diabetes. © 2015 The Authors.EnglishAll Open Access; Gold Open Access; Green Open AccessAnimalsAntioxidantsCarnitineDiabetes Mellitus, ExperimentalDiabetes Mellitus, Type 2Diet, High-FatDyslipidemiasEndothelium, VascularGlutathione PeroxidaseHumansLiverMelatoninNitric OxideOxidative StressRatsSuperoxide DismutaseTriglyceridesacetylcholinecarnitineglibenclamideglucoseglutathione peroxidasehigh density lipoprotein cholesterolinsulinlow density lipoprotein cholesterolmalonaldehydemelatoninnitric oxidenitroprusside sodiumsuperoxide dismutasetriacylglycerolantioxidantcarnitineglutathione peroxidasemelatoninnitric oxidesuperoxide dismutasetriacylglycerolanimal experimentanimal modelanimal tissueantioxidant activityArticlecholesterol blood levelcontrolled studydiet restrictionendothelial dysfunctionenzyme blood levelglucose blood levelinsulin blood levellipid dietlipid peroxidationmalenon insulin dependent diabetes mellitusnonhumanoxidative stresspriority journalratstreptozotocin-induced diabetes mellitusvasodilatationanimalblooddrug effectsdyslipidemiaenzymologyexperimental diabetes mellitushumanlivermetabolismnon insulin dependent diabetes mellitusoxidative stresspathologyvascular endotheliumArticle10.1016/j.redox.2015.11.007