Cirpan T.Akercan F.Terek M.C.Kazandi M.Ozcakir H.T.Giray G.Sagol S.2024-07-222024-07-22200703906663http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19072Objective: The aim of the study was to determine VEGF protein with immunohistochemical staining in placental bed biopsies of preeclamptic pregnancies in comparison to normal controls. Design: Prospective cohort study. Methods: The placental bed biopsies were obtained from 12 patients with preeclapmsia and ten patients for a control group at the time of cesarean delivery. Tissue samples of the placental bed were examined for VEGF protein distribution with avidin-biotin-peroxidase immunohistochemistry. Two blinded histopathologists were asked to score each sample for the intensity of staining and the number of cells stained in a randomly selected HPF of each sample. The resulting "H-score" was computed as a product of intensity and percent of cells stained. Results: VEGF expression was significantly lower in both the myometrium and stroma of the preeclamptic group compared to the control group (77.2 ± 25.4 vs 134 ± 44.3, p = 0.007; 194.1 ± 20.7 vs 170.2 ± 17, p = 0.017, respectively). Conclusion: VEGF expression is significantly lower in placental bed biopsies of preeclamptic pregnancies.EnglishAdultCase-Control StudiesCesarean SectionFemaleHumansImmunohistochemistryPlacentaPre-EclampsiaPregnancyProspective StudiesVascular Endothelial Growth Factor Aavidinbiotinperoxidasevasculotropinadultarticlebiopsycell countcesarean sectionclinical articlecohort analysiscomparative studycontrol groupcontrolled studyfemalehistopathologyhumanhuman tissueimmunohistochemistrymyometriumphysicianplacentapreeclampsiapregnancyprospective studyprotein determinationprotein expressionprotein localizationsamplescoring systemstainingstromaArticle