Kilciksiz S.Demirel C.Ayhan S.E.Erdal N.Gurgul S.Tamer L.Ayaz L.2024-07-222024-07-22201111070625http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18190Purpose: The present study was designed to investigate the potential radioprotective effects of N-acetylcysteine (NAC) on radiation-induced nitrosative stress caused by gamma irradiation (single dose, 6 Gy) in rat liver. Methods: The rats (n=40) were divided randomly and equally into 4 groups: Control (C), Radiation (R), R+NAC (received irradiation and 1,000 mg/kg of NAC) and R+WR-2721 (received irradiation and 200 mg/kg of WR-2721). Liver tissue of each animal was harvested and utilized for 3-nitrotyrosine (3-NT) detection using high-performance liquid chromatography-ultraviolet (HPLC-UV) system. Results: In the R rats, 3-NT levels significantly increased when compared to those of the C rats (p<0.05). There were no significant differences in the 3-NT levels among R+NAC and R+WR-2721 rats. Histologically examined liver tissue samples showed no obvious differences. Conclusion: The present study suggests that irradiation has a negative effect on the cellular proteins by enhancing 3-NT formation. The prophylactic use of NAC seems to reduce the nitrosative damage during radiotherapy. © 2011 Zerbinis Medical Publications.EnglishAcetylcysteineAmifostineAnimalsFemaleLiverNitric OxideRadiation-Protective AgentsRatsRats, WistarTyrosine3 nitrotyrosineacetylcysteineamifostinetyrosineanimal cellanimal experimentanimal tissuearticlecontrolled studygamma irradiationhigh performance liquid chromatographyliver cellliver histologyliver injurynitrosative stressnonhumanradiation doseradiation protectionrattreatment outcomeArticle