Aydemir T.Kavrayan D.2024-07-222024-07-22200915324184http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18866The glutathione-s-transferases are a family of multifunctional enzymes involved in the detoxification of electrophilic xenobiotics primarily through conjugation to reduce glutathione. A form of the enzyme, designated GSH-S transferase ρ, was purified chicken erythrocyte by acetone precipitation, ethanol-chloroform treatment, DEAE-Cellulose, Q-Sepharose, Sephadex G-100 chromatography. The molecular weight of GST purified from chicken erythrocyte was estimated as 47,500 Da by gel filtration. The subunit molecular weight of chicken erythrocyte GST as determined by electrophoresis in the presence of sodium dodecyl sulfate was predicted as 24,000 Da. The specific activity was found to be 20.39 U/mg. The km for CDNB calculated from Lineweaver-Burk plot was 0.71 mM. Optimum temperature of maximum GST activity was 28°C for CDNB. The maximal activity of the enzyme was observed at pH 7.5. The activity of purified GST is inhibited by DDT, urea, CDNB, Triton X-100, DTNB. Copyright © 2009 Informa UK Ltd.EnglishAll Open Access; Bronze Open AccessAcetoneAnimalsChickensChromatography, DEAE-CelluloseDDTDithionitrobenzoic AcidEnzyme ActivationErythrocytesGlutathione TransferaseHydrogen-Ion ConcentrationMolecular WeightOctoxynolPrecipitationTemperatureUreaAcetoneBiochemistryChromatographic analysisDetoxificationElectrophoresisEnzyme activityEthanolGel permeation chromatographyGelationIon exchangeMeatsMolecular weightPurificationSodiumSodium sulfateSystem theoryUreaalcoholchloroformdiethylaminoethyl cellulosedodecyl sulfate sodiumglutathione transferasesephadexsepharoseChicken erythrocyteChloroform treatmentsGel filtrationsGlutathioneGlutathione-S-transferaseInhibitorsLineweaver-Burk plotsOptimum temperaturesQ-sepharoseSephadexSodium dodecyl sulfatesSpecific activitiesTriton x-100acid precipitationanimal cellarticlechromatographydetoxificationelectrophoresisenzyme activityenzyme analysisenzyme purificationenzyme specificityerythrocytegel filtrationmolecular weightnonhumanpolyacrylamide gel electrophoresispredictionprotein familyxenobiotic metabolismEnzymesArticle10.1080/10731190902742489