Wang, LKaya, HBZhang, NRai, RWillmann, MRCarpenter, SCDRead, ACMartin, FFei, ZJLeach, JEMartin, GBBogdanove, AJ2025-04-102025-04-10http://hdl.handle.net/20.500.14701/42335Prime editing is an adaptation of the CRISPR-Cas system that uses a Cas9(H840A)-reverse transcriptase fusion and a guide RNA amended with template and primer binding site sequences to achieve RNA-templated conversion of the target DNA, allowing specified substitutions, insertions, and deletions. In the first report of prime editing in plants, a variety of edits in rice and wheat were described, including insertions up to 15 bp. Several studies in rice quickly followed, but none reported a larger insertion. Here, we report easy-to-use vectors for prime editing in dicots as well as monocots, their validation in Nicotiana benthamiana, rice, and Arabidopsis, and an insertion of 66 bp that enabled split-GFP fluorescent tagging.EnglishArticle2673-3439