Ozbilgin M.K.Karaman G.Z.Gencur S.Gumustepe E.Kurtman C.2024-07-222024-07-22202023223243http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13921Background: Adrenomedullin (AM) and its receptor, receptor actvity-modifying protein (RAMP) 2 have pleiotropic regulatory functons in normal tssue and cancer tssue. AM is produced and secreted both numerous stromal cells and tumor cells. This study aims to investgate a possible role of AM and RAMP2 in the radiaton exposure in the normal lung tssue. M aterial s and Methods: Four groups with 6 male adult Swiss Albino mice per group were investgated. The mice were subjected to a 500 cGy single-dose radiaton exposure in the total body radiaton device and lung tssues were collected. 1, 2, and 7 days after radiaton exposure, with 1 reference group which was not exposed to radiaton. Results: The general histology and the immunohistochemistry of the tssue samples prepared with ant-AM, ant-RAMP2, and monoclonal antbodies were investgated, yielding a statstcally significant increase for AM on day 3 and for RAMP2 on day 1 after radiaton exposure. Conclusion: The observed increase of AM and RAMP2 concentratons in the normal tssue matrix after radiaton exposure may play a role in the side effects of radiotherapy. © 2020 Novin Medical Radiation Institute. All rights reserved.Englishadrenomedullinketaminemonoclonal antibodyreceptor activity modifying protein 2adultanimal experimentanimal modelanimal tissueArticleconnective tissuecontrolled studyfibroblasthistologyimmunohistochemistryimmunoreactivitylung injurylung parenchymamacrophagemalemousenonhumanprotein expressionradiation doseradiation exposurestroma cellwhole body radiationArticle10.18869/acadpub.ijrr.18.3.571