Polatoğlu İ.Yardım A.2024-07-222024-07-22202200032697http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12731Heavy metal detection is critical due to its harmful effects on human health and the ecosystem. Enzyme-based platforms attract attention for heavy metal detection such as silver, a toxic metal, due to being small, portable, and requiring only essential equipment compared with the basic analytical methods. In this study, magnetic cross-linked invertase aggregates (MCLIA) were developed for the first time as an enzyme-based signaling platform to detect Ag+ using a personal glucose meter (PGM). EDX, FTIR, and VSM results depicted that MCLIA was successfully developed and exhibits super-paramagnetism. In addition, MCLIA selectively detected the Ag+ at a sensitivity of 1.2 inhibition rate/μM in a linear range from 5 to 70 μM with a detection limit of 4.6 μM and IC50 value of 42.3 μM. These findings strongly indicate that MCLIA is applicable as a signal platform for portable quantification of other analytes that inhibits the invertase enzyme. © 2021 Elsevier Inc.EnglishBiosensing TechniquesBlood Glucose Self-MonitoringGlucose OxidaseIonsSilverAggregatesChemical detectionEnzyme inhibitionMagnetiteMetal ionsSilvercobaltcoppermagnetite nanoparticlenickelsilverzincglucose oxidaseionsilverAg +Ag+ detectionEnzyme aggregateHarmful effectsHeavy metals detectionHuman healthInvertase inhibitionPersonal glucose meterPortable quantificationSilver ionsanalytic methodanalytical parametersArticlecalibration curvechemical compositionchemical structureenergy dispersive X ray spectroscopyenzyme activityenzyme inhibitionFourier transform infrared spectroscopyIC50immobilizationlimit of detectionlinearitymagnetite cross linked invertase aggregate methodscanning electron microscopysensitivityvibrating sample magnetometryblood glucose monitoringgenetic proceduresmetabolismGlucoseArticle10.1016/j.ab.2021.114527