Tepe M.Zeybek M.S.2024-07-222024-07-22202400084042http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/11335In this study, silver nanostructures (AgNSs) were obtained using aqueous extracts of elecampane (Inula viscosa) andblue berry (Vaccinium arctostaphylos L.). The synthesized AgNSs were characterized using UV–visible spectrophotometers, Fourier-transformed infrared spectroscopy, scanning electron microscopy-energy dispersive X-ray analysis, high-resolution transmission electron microscopy, and X-ray photoelectron spectroscopy. The optimization studies revealed that pH and the extract/AgNO3 ratio played significant roles in the morphology and size of the AgNSs, while temperature did not show any significant role. Both the blueberry and elecampane extracts resulted in a mixture of spherical and aspherical silver nanoparticles (AgNPs). In contrast, utilization of a relatively high extract/AgNO3 ratio accompanied by an extended incubation period (i.e., 10 days) caused the formation of larger spherical (e.g., up to micron-sized) and large 1D AgNPs for the blueberry and elecampane extracts, respectively. The major difference related to the crystal structures was obtained, as the blueberry extracts resulted in face-centric cubic crystals, and the elecampane extracts allowed the formation of hexagonally close-packed (hcp) crystalline structures. The AgNPs were tested for their antibacterial activities using gram (−) andgram(+) bacterial species, where the elecampane-synthesized AgNPs possessed higher antibacterial activity that can be attributed to the hcp crystalline structure of the AgNPs. © 2024 The Author(s).EnglishEnergy dispersive X ray analysisHigh resolution transmission electron microscopyInfrared spectroscopyPlant extractsScanning electron microscopySilver compoundsSilver nanoparticlesX ray diffraction analysisX ray photoelectron spectroscopyAnti-bacterial activityAqueous extractsBlueberryClose packedCrystalline structureCrystals structuresElecampaneGreen synthesisSilver nanostructuresSynthesisedCrystal structureArticle10.1139/cjc-2023-0220