Deliormanlı A.M.Türk M.Atmaca H.2024-07-222024-07-22201808853282http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14845In the study, three-dimensional, grid-like silicate-based bioactive glass scaffolds were manufactured using a robotic deposition technique. Inks were prepared by mixing 13-93 bioactive glass particles in Pluronic® F-127 solution. After deposition, scaffolds were dried at room temperature and sintered at 690°C for 1 h. The surface of the sintered scaffolds was coated with graphene nanopowder (1, 3, 5, 10 wt%) containing poly(ε-caprolactone) solution. The in vitro mineralization ability of the prepared composite scaffolds was investigated in simulated body fluid. The surface of the simulated body fluid-treated scaffolds was analyzed using scanning electron microscopy to investigate the hydroxyapatite formation. Mechanical properties were tested under compression. Results revealed that graphene coating has no detrimental effect on the hydroxyapatite forming ability of the prepared glass scaffolds. On the other hand, it decreased the compression strength of the scaffolds at high graphene concentrations. The prepared grid-like bioactive glass-based composite scaffolds did not show toxic response to bone marrow mesenchymal stem cells. It was shown that stem cells seeded onto the scaffolds attached and proliferated well on the surface. Cells seeded on the scaffolds surface also demonstrated osteogenic differentiation under in vitro conditions in the absence of transforming growth factors. © The Author(s) 2018.EnglishAnimalsBiocompatible MaterialsCell AdhesionCell ProliferationCompressive StrengthDurapatiteGlassGraphiteMesenchymal Stem CellsMiceNanoparticlesOsteogenesisPoloxamerPolyestersSilicatesTissue ScaffoldsBioactive glassBiomechanicsBody fluidsBoneCell cultureDepositionGrapheneHydroxyapatiteScaffoldsScanning electron microscopySilicatesSinteringStem cellsgraphenehydroxyapatitepolycaprolactone13-93 bioactive glassbiomaterialglassgraphitenanoparticlepoloxamerpolyestersilicateBone marrow mesenchymal stem cellsGlass-based compositesHydroxyapatite formationsMesenchymal stem cellOsteogenic differentiationPoly (epsiloncaprolactone)RobocastingTransforming growth factorsanimal cellArticlebone marrow derived mesenchymal stem cellcell adhesioncell differentiationcell proliferationcell structurecell viabilitychemical structurecompressioncompressive strengthconcentration (parameters)controlled studycytotoxicitygrid like bioactive glass scaffoldin vitro studymaterial coatingmineralizationmousenonhumanporositypriority journalrobocastingroboticsscanning electron microscopysimulationanimalbone developmentchemistrycytologymesenchymal stem celltissue scaffoldScaffolds (biology)Article10.1177/0885328218799610