Eskiizmir G.Çalıbaşı Koçal G.Uysal T.Ellidokuz H.Başpınar Y.2024-07-222024-07-22202117494478http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13160Objectives: To determine the serum hypoxia-inducible factor-1, -2 and -3 (HIF-1, -2 and -3) levels in patients with laryngeal neoplasm, and to investigate their role in differential diagnosis, prediction of tumour characteristic and extension, and prognosis and survival. Study Design: Prospective, cohort study at a tertiary referral centre. Settings: The study was conducted in a tertiary medical centre. Participants: Patients with benign, premalignant and malignant laryngeal neoplasms were included. Sixty-four patients with a laryngeal neoplasm were enrolled. Main Outcome Measures: Serum HIF-1, -2 and -3 levels were measured from blood samples that were drawn before treatment, using ELISA. Results: A statistically significant difference between benign (HIF-1, -2, -3:4046,1 pg/mL; 2581,5 pg/mL; 1321,0 pg/mL), premalignant (HIF-1, -2, -3:3630,3 pg/mL; 3229,7 pg/mL; 2549,8 pg/mL) and malignant (HIF-1, -2, -3:3576,7 pg/mL; 2595,8 pg/mL; 1106,3 pg/mL) laryngeal neoplasms was not detected when serum HIF-1, -2 and -3 levels were compared. However, high serum HIF-2 level adversely affected survival and locoregional control and had more than 7-fold increase in hazard ratio. Moreover, serum HIF-2 was an independent prognostic factor for 2-year overall, disease-free, distant metastasis-free survival and locoregional control. Conclusion: This is the first clinical study in which the diagnostic, predictive and prognostic roles of hypoxia-related biomolecules were examined in laryngeal neoplasms. Hypoxia-inducible factor-2 is a prognostic factor in larynx cancer irrespective of treatment modality. © 2021 John Wiley & Sons Ltd.EnglishAdultAgedAged, 80 and overBasic Helix-Loop-Helix Transcription FactorsBiomarkers, TumorCohort StudiesDisease-Free SurvivalFemaleHumansHypoxiaLaryngeal NeoplasmsMaleMiddle AgedPrognosisProspective Studieshypoxia inducible factorhypoxia inducible factor 1hypoxia inducible factor 2hypoxia inducible factor 3tumor markerunclassified drugbasic helix loop helix transcription factortumor markeradultanemiaArticlecancer prognosiscancer survivalclinical featurediagnostic valuedifferential diagnosisdisease associationdisease free survivaldistant metastasis free survivalenzyme linked immunosorbent assayfemalehumanlarynx cancermajor clinical studymaleoverall survivalpredictive valueprospective studyprotein blood levelprotein functionsurvival timetertiary care centertumor invasionagedbloodcohort analysishypoxialarynx tumormiddle agedprognosisvery elderlyArticle10.1111/coa.13789