Atmaca H.Bozkurt E.2024-07-222024-07-22201610104283http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15967Plants, due to their remarkable composition, are considered as natural resources of bioactive compounds with specific biological activities. Salvia genus (Lamiaceae) has been used around the world in complementary medicine since ancient times. We investigated the cytotoxic, apoptotic and anti-angiogenic effects of methanolic Salvia triloba extract (STE) in prostate cancer cells. Cell viability was evaluated by XTT; apoptosis was investigated by DNA fragmentation and caspase 3/7 activity assays. Changes in the angiogenic cytokine levels were investigated by human angiogenesis antibody array. Scratch assay was used to determine the cell motility. STE induced cytotoxicity and apoptosis in a concentration-dependent manner in both cancer cells; however, it was not cytotoxic to normal cells. Cell motility was reduced in PC-3, DU-145 and HUVEC cells by STE treatment. ANG, ENA-78, bFGF, EGF, IGF-1 and VEGF-D levels were significantly decreased by −2.9, −3.7, −1.7, −1.7, −2.0 and −1.8 fold in STE-treated DU-145 cells, however, ANG, IL-8, LEP, RANTES, TIMP-1, TIMP-2 and VEGF levels were significantly decreased by −5.1, −2.0, −2.4, −3.1, −1.5, −2.0 and −2.5 fold in PC-3 cells. These data suggest that STE might be a promising candidate for anti-tumor and anti-angiogenic treatment of prostate cancer. © 2015, International Society of Oncology and BioMarkers (ISOBM).EnglishAngiogenesis InhibitorsApoptosisBlotting, WesternCell LineCell Line, TumorCell MovementCell ProliferationCell SurvivalDose-Response Relationship, DrugDrugs, Chinese HerbalFluorescent Antibody TechniqueHumansMaleNeovascularization, PathologicProstatic NeoplasmsSalviaTime FactorsVascular Endothelial Growth Factor Aangiogenesis inhibitorbasic fibroblast growth factor receptorcaspase 3caspase 7epidermal growth factorepithelial derived neutrophil activating factor 78interleukin 8plant extractRANTESSalvia triloba extractsomatomedin Ctissue inhibitor of metalloproteinase 1tissue inhibitor of metalloproteinase 2unclassified drugvasculotropin Dangiogenesis inhibitorherbaceous agentSalvia extractvasculotropin Aantiangiogenic activityapoptosisArticlecell motilitycell viabilityconcentration responsecontrolled studyDNA fragmentationdrug effectenzyme activityenzyme assayhumanhuman cellmalepriority journalprostate cancer cell lineSalviaSalvia trilobaumbilical vein endothelial cellapoptosiscell linecell motioncell proliferationcell survivalchemistrydose responsedrug effectsfluorescent antibody techniquemetabolismNeovascularization, Pathologicpathologyprostate tumortime factortumor cell linevascularizationWestern blottingArticle10.1007/s13277-015-4208-2