Atmaca H.Oğuz F.Ilhan S.2024-07-222024-07-22202203034569http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12391Trabectedin is a chemotherapy agent originating from a tunicate, Ecteinascidia turbinata. In this study, DNA-independent action mechanisms of trabectedin are investigated in prostate cancer (PCa) cells. Cell viability was assessed via XTT assay. Apoptosis was evaluated via flow cytometry. Tetramethylrodamine ethyl ester (TMRE) dye was utilized to determine mitochondrial membrane potential (MMP). Cell cycle distribution was investigated via flow cytometric analysis. Reactive oxygen species (ROS) were monitored using fluorescence CM-H2DCFDA dye. Changes in CHOP, p-eIF2α, GRP78 and p-PERK which are endoplasmic reticulum (ER) stress-involved proteins were investigated via western blot. Trabectedin induced cytotoxicity and cell cycle arrest at the G2/M phase. Trabectedin decreased MMP via ROS generation in PCa cells. ER stress-related proteins CHOP, p-eIF2α, GRP78 and p-PERK were also elevated by trabectedin treatment indicating the induction of ER stress-induced apoptosis. The results of this study show that trabectedin may be an effective chemotherapeutic for PCa. © 2022 Wiley-VCH GmbH.EnglishAll Open Access; Gold Open AccessApoptosisCell Line, TumorEndoplasmic Reticulum StressHumansMaleProstatic NeoplasmsReactive Oxygen SpeciesTrabectedinendoplasmic reticulum chaperone BiPfluorescent dyegrowth arrest and DNA damage inducible protein 153initiation factor 2alphamatrix metalloproteinasemitogen activated protein kinasereactive oxygen metabolitetetramethylrodamine ethyl estertrabectedinunclassified drugreactive oxygen metabolitetrabectedinapoptosisapoptotic cell percentageArticlecancer inhibitioncell cycle arrestcell cycle G1 phasecell viabilitydrug mechanismDU145 cell lineendoplasmic reticulum stressflow cytometryG2 phase cell cycle checkpointHEK293 cell linehumanhuman cellIC50mitochondrial membrane potentialoxidative stressPC-3 [Human prostate carcinoma] cell lineprostate cancerprotein expressionWestern blottingXTT assayapoptosismalemetabolismprostate tumortumor cell lineArticle10.1111/and.14599