Oztatlici M.Ozdemir A.T.Oztatlici H.Kucukhuyuk S.Ozgul Ozdemir R.B.Orhan H.Ozbilgin K.2024-07-222024-07-22202425872400http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/11671Objectives: The aim of this study is to investigate the immunomodulatory effects of MDA-MB-231 cells or MDA-MB-231-derived exosome-mimetic nanovesicles (NVs) on CD4+ Jurkat cells. Methods: NVs were produced by the breakdown of MDA-MB-231 cells and the characterization of generated NVs were performed by using direct-ELISA and Flow cytometry methods. We co-cultured CD4+ Jurkat cells with MDA-MB-231 cells or MDA-MB-231-derived NVs for 48 h. Subsequently, expressions of pro-inflammatory and anti-inflammatory cy-tokines, and related transcription factors of CD4+ Jurkat cells were evaluated by qPCR method. Results: Clustering, which is the indicator of activation, was not seen in the CD4+ Jurkat cells co-cultured with MDA-MB-231 cells. However, CD4+ Jurkat cell clusters were observed in the co-culture experiments with all NV concentrations. In addition, it was determined that the expressions of pro-inflammatory cytokines significantly increased while the expressions of anti-inflammatory cytokines was dramatically decreased in the NV-treated groups. On the contrary, opposite results were obtained in CD4+ Jurkat cells co-cultured with MDA-MB-231 cells. Moreover, TNF-α and Gata3 expressions were decreased in all groups. Conclusion: These preliminary findings from in-vitro experiments suggested that NVs could be a potential tool in cancer immunotherapy, but our data need to be supported by more comprehensive studies. © 2024 by Eurasian Journal of Medicine and Oncology.EnglishAll Open Access; Gold Open Accesscytokinegamma interferoninterleukin 10interleukin 17interleukin 4transcription factor FOXP3transcription factor GATA 3transforming growth factor beta1tumor necrosis factorArticleCD4+ T lymphocytecell activationcell culturecell isolationdirect ELISAexosomeflow cytometrygene expressionhumanhuman cellimmunomodulationJurkat cell lineMDA-MB-231 cell lineprotein expressionprotein functionArticle10.14744/ejmo.2024.64067