Becer E.Kabadayi H.Meriçli A.H.Kivançli B.Vatansever H.S.Meriçli F.2024-07-222024-07-22202111298723http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13434Opuntia ficus-indica belongs to the family Cactaceae that particularly rich in unsaturated fatty acids. The aim of this study was to investigate the composition and in vitro anti-angiogenic activity of spiny and thornless Opuntia ficus-indica seed (CPS) oils. Spiny and thornless CPS oils were obtained by supercritical CO2 extraction and analyzed by GC-MS. Cell growth and cytotoxicity were measured with MTT assay with different concentrations of spiny and thornless CPS oils in Colo-320 and Colo-741 cell lines. Anti-angiogenic activity of CPS oils were investigated by immunocytochemistry using antibodies directed against to Flt- 1, Flk-1, eNOS, iNOS, IL-6, PGE2, TNF-α and VEGF. Oleic acid (17.6%; 15.6%), linoleic acid (55.9%; 60.1%), palmitic acid (12.4%; 12.3%), elaidic acid (4%; 4.1%) were determined as the major compounds of spiny and thornless CPS oils, respectively. In the MTT assay, 1:16 dilution spiny CPS oil was found to be active against Colo-320 and Colo-741 cells for 48h incubation. Also, 1:8 and 1:16 dilutions of thornless CPS oil were more effective at inhibiting Colo-320 and Colo-741 for 48 h, respectively.We conclude that spiny CPS oil decreased signaling molecules which play in PGE2 mediated and VEGF-dependent angiogenesis. Interestingly, thornless CPS oil increased angiogenesis thought signaling molecules in colon cancer cells. © 2021 Mattioli 1885. All rights reserved.Englishelaidic acidendothelial nitric oxide synthasefatty acidinducible nitric oxide synthaseinterleukin 6Ki 67 antigenlinoleic acidoleic acidpalmitic acidplant extractprostaglandin E2tumor necrosis factorvasculotropinvasculotropin receptor 1vasculotropin receptor 2vegetable oilantiangiogenic activityantigen expressionantineoplastic activityantiproliferative activityArticlecancer inhibitioncell growthcell proliferationCOLO 320 cell linecolon carcinomacolon carcinoma cell lineconcentration (parameter)controlled studydilutiondrug cytotoxicitydrug determinationdrug identificationdrug isolationdrug mechanismhumanhuman cellimmunocytochemistryin vitro studylipid compositionmass fragmentographymigration inhibitionMTT assayOpuntia ficus-indicaArticle10.23751/pn.v23i2.10042