Dilber Y.Inan S.Ercan G.A.Sencan A.2024-07-222024-07-22201600651281http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16100Ischemia reperfusion injury arises from testicular torsion resulting in a loss of spermatogenesis and significant germ cell apoptosis. This study evaluates the prooxidant/antioxidant effects of caffeic acid phenethyl ester (CAPE) through PI3K/AKT/mTOR signal pathways on testis torsion. A total of (28) male Wistar rats were divided randomly into 4 groups (n= 7 for each group):group A (sham) group,group B torsion/detorsion group, group C (saturation group, during four days of CAPE, one dose (10. μmol/kg, i.p)) and group D (a single dose of CAPE 2 h after torsion and before detorsion). At the end of the study, unilateral orchiectomies were performed for measurements of MDA and 8OHdG levels, histopathologic and immunohistochemical and TUNEL apoptotic cell examination. Testicular torsion-detorsion led to a significant decrease in the mean values of the Johnsen's scores and a significant increase in the apoptotic cell values of group B. There were no significant differences between group D and group A. In addition, the MDA and 8OHdG levels increased significantly in group B. The MDA and 8OHdG values were lower in group D. However, the 8OHdG levels were higher in group C than the groups A and D. On the other hand, CAPE suppresses mTOR activation and reduces the apoptosis on ischemia/reperfusion damage in rat testis. These results demonstrate that CAPE suppresses mTOR activation and reduces the apoptosis on ischemia/reperfusion damage in rat testis. © 2015 Elsevier GmbH.EnglishAnimalsAntioxidantsCaffeic AcidsDrug Evaluation, PreclinicalMaleOxidative StressPhenylethyl AlcoholPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktRats, Sprague-DawleySignal TransductionSpermatic Cord TorsionTestisTOR Serine-Threonine Kinases8 hydroxydeoxyguanosinecaffeic acid phenethyl estermalonaldehydemammalian target of rapamycinphosphatidylinositol 3 kinaseprotein kinase Bantioxidantcaffeic acid derivativecaffeic acid phenethyl estermTOR protein, ratphenethyl alcoholphosphatidylinositol 3 kinaseprotein kinase Btarget of rapamycin kinaseanimal experimentanimal modelanimal tissueantioxidant activityapoptosisArticlecontrolled studyenzyme activationhistopathologyimmunohistochemistrymalenonhumanorchiectomyoxidative stressprotein functionratreperfusion injurysignal transductionsingle drug dosetestis detorsiontestis detorsiontestis diseasetestis torsionTUNEL assayanalogs and derivativesanimaldrug effectsmetabolismpathologypreclinical studySprague Dawley rattestistestis torsionArticle10.1016/j.acthis.2015.11.004