Micili S.C.Goker A.Sayin O.Akokay P.Ergur B.U.2024-07-222024-07-22201315672387http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17330Aim of this study was to investigate the effects of lipoic acid on uterine wound healing by immunohistochemical and biochemical assay in a rat uterine horn model with full thickness injury. Thirty-two female Wistar albino rats were randomised into five groups: Control group, with no intervention; uterine scar group 15days (US15d), uterine scar group 15 days + alpha lipoic acid (ALA) (US15d + ALA), uterine scar group 30 days (US30d) and uterine scar group 30 days + ALA (US30 days + ALA). After uterine incision 100 mg/kg of ALA was administered by oral gavage for either 15 or 30 days. Vascular endothelial growth factor (VEGF) and alpha smooth muscle actin (α-SMA) distribution were evaluated by immunohistochemical methods in tissue and ELISA methods in tissue homogenate. The percentage of α-SMA positive area in US15d + ALA and US30d + ALA groups was significantly higher than US15 and US30d groups. The percentage of VEGF positive area in US15d + ALA group was significantly higher than US15d group and US30d + ALA group was significantly higher than US30d group. Biochemically, α-SMA was significantly higher in the US15d + ALA group when compared to US15d group and higher in US30d + ALA group when compared to US30d group. VEGF was significantly higher in US15d + ALA and US30d + ALA groups when compared to US15 and US30d groups. In conclusion, ALA was found to be effective in enhancing wound healing in uterine full thickness injury. © 2013 Springer Science+Business Media Dordrecht.EnglishActinsAnimalsCicatrixFemaleMuscle, SmoothNeovascularization, PhysiologicRatsRats, WistarRegenerationThioctic AcidUterusVascular Endothelial Growth Factor AWound HealingRattusalpha smooth muscle actinthioctic acidvasculotropinangiogenesisanimal experimentanimal modelanimal tissuearticlecontrolled studydrug efficacydrug mechanismenzyme linked immunosorbent assayfemalefull thickness uterine injuryhistopathologyimmunohistochemistrymousemuscle regenerationnonhumanpriority journalprotein localizationscarsmooth muscletreatment durationtreatment outcomeuterus diseasewound healingArticle10.1007/s10735-013-9485-8