Tanriverdi S.Polat M.Onay H.2024-07-222024-07-22202113110160http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13118Cystic fibrosis (CF) is an autosomal recessive disease. The genetic transition occurs with CF transmembrane conductance regulator (CFTR) gene mutation. We aimed to determine the frequency of CF mutations and also new mutations in the CFTR gene in neonates with respiratory distress. Newborn babies hospitalized due to respiratory distress were included in the patient group. The control group consisted of infants who had no respiratory distress. The CFTR genes of both groups were analyzed using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods. A total of 40 patients (20 in the patient group and 20 in the control group) were evaluated. The CFTR gene analysis was normal in 16 neonates in the patient group, whereas in others: A46D (c.137C>A) (n = 1), D1312G (c.3935A>G) (n = 1), R117H (c.350G>A) (n = 1), S1426P (c.4276T>C) (n = 1) heterozygotes were detected; CFTR gene analysis was normal at 14 neonates in the control group, whereas in others: E1228G (c.3683A>G) (n = 1), E217G (c.650A>G) (n = 1), E632TfsX9 (c1894_1895delAG) (n = 1), I807M (c.2421 A>G) (n = 2), S573F (c.1718C>T) (n = 1) heterozygotes were detected. There was no significant difference in the patient and control groups' CFTR gene analysis (p = 0.340). This study demonstrates the importance of CFTR gene analysis in asymptomatic newborn infants for follow-up and early diagnosis of CFTR-related disorders. In this study, a c.1894_1895delAG (E632TfsX9) heterozygous mutation detected in the CFTR gene in an asymptomatic newborn infant, was first encountered in the literature. © 2021 S Tanriverdi et al., published by Sciendo.EnglishAll Open Access; Gold Open Access; Green Open AccessbicarbonateC reactive proteincystic fibrosis transmembrane conductance regulatorlung surfactantsteroidApgar scoreArticleartificial ventilationasymptomatic diseasebicarbonate blood levelblood carbon dioxide tensionbrain hemorrhagecapillary electrophoresisclinical articlecontrolled studycoughingcracklecystic fibrosisdehydrationdemographicsDNA isolationearly diagnosisexonfemalefollow upgene amplificationgene frequencygene mutationgenetic analysisgestational ageground glass opacityheterozygosityhospitalized infanthumanhyperbilirubinemiahypernatremic dehydrationintrauterine growth retardationlaboratory testlength of staylung auscultationlung dysplasialung infiltratemalematernal agemetabolic acidosismuscle hypotoniamutation rateneonatal intensive care unitneonatal pneumonianeonatal respiratory distress syndromenewbornnewborn apneanewborn sepsisoxygen consumptionoxygen therapypatent ductus arteriosusplatelet countpneumoniapolymerase chain reactionprematuritypulmonary hypertensionrestriction fragment length polymorphismSanger sequencingsequence analysistachycardiatachypneathrombocytopeniawheezingArticle10.2478/bjmg-2021-0023