Atmaca H.Camli Pulat C.Cittan M.2024-07-222024-07-22202209603123http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13079Liquidambar orientalis Mill (LOM), is an endemic species having a local distribution in the southwestern coastal district of Turkey. Styrax liquidus gum (SLG), is a gum-like resinous which exudates in response to injury of the trunk of LOM. The aim of the study was to investigate the cytotoxic effects and the molecular mechanisms of the ethanolic SLG extract in human prostate cancer cells. GC-MS analysis was performed to identify the volatile compound composition. Cytotoxicity was determined by XTT analysis. Apoptosis and necrosis were evaluated via ELISA assay. Autophagic cell death was detected via monodansylcadaverine (MDC) staining and by measuring the levels of LC3I and LC3II. The protein levels of p-PI3K, p-Akt and p-mTOR were evaluated by western blot analysis. In the present study, it is shown that the SLG extract containing a considerable amount of ravidomycin derivate induced autophagic cell death in prostate cancer cells via inhibiting the PI3K/Akt/mTOR pathway. © 2020 Informa UK Limited, trading as Taylor & Francis Group.EnglishApoptosisAutophagyHumansLiquidambarMalePhosphatidylinositol 3-KinasesPlant ExtractsProstatic NeoplasmsProto-Oncogene Proteins c-aktSignal TransductionTOR Serine-Threonine KinasesTurkeyalcoholcytotoxic agentdansylcadaverinelc3i proteinlc3ii proteinLiquidambar orientalis extractmammalian target of rapamycinphosphatidylinositol 3 kinaseplant extractproteinprotein kinase Bunclassified drugvolatile agentMTOR protein, humanphosphatidylinositol 3 kinaseplant extractprotein kinase Btarget of rapamycin kinasecancercellendemic speciestoxicityAkt signalingapoptosisArticleautophagic cell deathcell viabilitycontrolled studycytotoxicityDU145 cell lineembryoenzyme linked immunosorbent assayhumanhuman cellIC50LiquidambarLiquidambar orientalismalemass fragmentographyPC-3 [Human prostate carcinoma] cell lineprostate cancertumor necrosisWestern blottingXTT assayautophagymetabolismprostate tumorsignal transductionArticle10.1080/09603123.2020.1818187