Deliormanlı A.M.2024-07-222024-07-22201902732289http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14409Scaffold and mesenchymal stem cell–based cartilage tissue engineering offers a favorable way for the repair and regeneration of injured cartilage. In this study, poly (ε-caprolactone) PCL scaffolds with grid-like structure having periodic lattice was manufactured by robocasting method in the presence of graphene nanoplatelets for cartilage tissue engineering applications. For this purpose, a PCL solution (20 wt%) containing pristine graphene nanopowders in the form of platelets was prepared as printing ink and it was dispensed through a nozzle at room temperature to an ethanol bath at 4 °C. The construction of porous scaffolds was made by a layer-by-layer assembly. Results revealed that graphene additions were not detrimental to deposition process and the structure of the resultant scaffolds. In vitro cell tests indicated that the prepared grid-like graphene/PCL composite scaffolds have good cytocompatibility and non-toxicity for mouse bone marrow mesenchymal stem cells. The stem cells attached and proliferated well on the scaffolds and they also demonstrated a chondrogenic differentiation in the absence of transforming growth factors. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.EnglishAnimalsGraphiteMesenchymal Stem CellsMicePolyestersTemperatureTissue EngineeringTissue ScaffoldsBoneCartilageCell cultureCell engineeringGrapheneScaffoldsStem cellsTissueTissue regenerationalcoholglycosaminoglycan polysulfategrapheneinkpolycaprolactonegraphitepolycaprolactonepolyesterCaprolactoneCartilage tissue engineeringChondrogenic differentiationLayer-by-layer assembliesMesenchymal stem cellPoly (epsiloncaprolactone)RobocastingTransforming growth factorsanimal cellArticlebone marrow derived mesenchymal stem cellcartilagecell adhesioncell differentiationcell proliferationcell structurecell viabilitychondrogenesiscontrolled studycytotoxicitydegradationdigital imagingin vitro studymousenonhumanroom temperaturescanning electron microscopytemperaturetissue engineeringanimalchemistrycytologymesenchymal stem cellprocedurestissue scaffoldScaffolds (biology)Article10.1007/s12010-019-02976-5