Degirmenci M.Erdogan A.P.Bulut G.Atmaca H.Uzunoglu S.Karaca B.Karabulut B.Uslu R.2024-07-222024-07-22201610104283http://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15966Prostate cancer (PCa) is the most common type of cancer among males. Although survival rate of early-stage PCa is high, treatment options are very limited for recurrent disease. In this study, the possible synergistic cytotoxic and apoptotic effect of octreotide in combination with AT-101 was investigated in DU-145 hormone and drug refractory prostate cancer cell line. To enlighten the action mechanisms of the combination treatment, expression levels of somatostatin receptors 2 and 5 (SSTR2 and SSTR5) were also investigated. Cell viability was measured by XTT assay. Apoptosis was assessed through DNA fragmentation analysis and caspase 3/7 assay. mRNA and protein levels of SSTR2 and SSTR5 were evaluated by qRT-PCR and western blot analysis, respectively. Octreotide in combination with AT-101 inhibited cell viability and induced apoptosis synergistically in DU-145 cells as compared to any agent alone. Combination treatment increased both SSTR2 and SSTR5 mRNA and protein levels in DU-145 cells. The data suggest that this combination therapy may be a good candidate for patients with advanced metastatic PCa do not respond to androgen deprivation. © 2015, International Society of Oncology and BioMarkers (ISOBM).EnglishAndrogensApoptosisCell Line, TumorCell ProliferationCell SurvivalDrug Resistance, NeoplasmDrug SynergismGene Expression Regulation, NeoplasticGossypolHumansMaleOctreotideProstatic NeoplasmsReceptors, Somatostatincaspase 3caspase 7isosorbidemessenger RNAoctreotidesomatostatin receptor 2somatostatin receptor 5androgengossypolgossypol acetic acidoctreotidesomatostatin receptorsomatostatin receptor 2somatostatin receptor 5antineoplastic activityArticlecell viabilitycontrolled studyDNA fragmentation assaydrug cytotoxicitydrug effectdrug mechanismDU 145 cancer cell lineenzyme assayhumanhuman cellmalepriority journalprostate cancer cell lineprotein determinationprotein functionquantitative analysisreverse transcription polymerase chain reactionanalogs and derivativesapoptosisbiosynthesiscell proliferationcell survivaldrug effectsdrug potentiationdrug resistancegene expression regulationgeneticsmetabolismpathologyProstatic Neoplasmstumor cell lineOctreotide in combination with AT-101 induces cytotoxicity and apoptosis through up-regulation of somatostatin receptors 2 and 5 in DU-145 prostate cancer cellsArticle10.1007/s13277-015-4331-0