Browsing by Author "Çöllü F."

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    The Effect of Nitric Oxide Synthase Inhibition in Developing Chick Embryo Lungs
    (Pleiades journals, 2022) Esra Ucar; Çöllü F.; Gürcü B.
    Abstract: We identified the developmental stages of the chicken lung, and explored the histopathological effects of L-nitro-arginine-methyl-ester (L-NAME) on such development at different times. L-NAME was injected into the vitellus, at two different doses, on the day of embryogenesis 6, 8, 9, 11, 13, and 16. Sham-injected and experimental embryos were collected on the day of incubation 7, 9, 10, 12, 14, and 17. Chicken lungs developed in four stages: embryonic (4–7 days), pseudoglandular (8–14 days), canalicular (14–17 days), and saccular (or alveolar) (day 17 and postnatally). Most differentiation occurred in the pseudoglandular stage. L-NAME triggered mesenchymal tissue loss, reduced airway branching and lung volume, narrowed bronchial diameters, triggered formation of pulmonary emboli, enhanced alveolar protein accumulation, caused regional bleeding (hemorrhage), triggered abnormal blood vessel modeling, and reduced vessel diameter. Sham-injected embryos exhibited strong immunoreactivities against endothelial and inducible nitrous oxide synthase in the embryonic and canalicular stages; immunoreactivities were reduced at all developmental stages in the experimental group. © 2022, Pleiades Publishing, Ltd.
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    Prion protein-dependent regulation of p53-MDM2 crosstalk during endoplasmic reticulum stress and doxorubicin treatments might be essential for cell fate in human breast cancer cell line, MCF-7
    (Elsevier Inc., 2023) Tuğrul B.; Balcan E.; Öztel Z.; Çöllü F.; Gürcü B.
    In this study, we investigated the effect of doxorubicin and tunicamycin treatment alone or in combination on MDM-, Cul9-and prion protein (PrP)-mediated subcellular regulation of p53 in the context of apoptosis and autophagy. MTT analysis was performed to determine the cytotoxic effect of the agents. Apoptosis was monitorized by ELISA, flow cytometry and JC-1 assay. Monodansylcadaverine assay was performed for autophagy. Western blotting and immunofluorescence were performed to determine p53, MDM2, CUL9 and PrP levels. Doxorubicin increased p53, MDM2 and CUL9 levels in a dose-dependent manner. Expression of p53 and MDM2 was higher at the 0.25 μM concentration of tunicamycin compared to the control, but it decreased at 0.5 μM and 1 μM concentrations. CUL9 expression was significantly decreased only after treatment of tunicamycin at 0.25 μM. According to its glycosylation status, the upper band of PrP increased only in combination treatment. In combination treatment, p53 expression was higher than control, whereas MDM2 and CUL9 expressions were decreased. Combination treatments may make MCF-7 cells more susceptible to apoptosis rather than autophagy. In conclusion, PrP may be important in determining the fate of cell death through crosstalk between proteins such as p53 and MDM2 under endoplasmic reticulum (ER) stress conditions. Further studies are needed to obtain in-depth information on these potential molecular networks. © 2023 Elsevier Inc.
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    Molecular analyses of ADAMTS-1, -4, -5, and IL-17 a cytokine relationship in patients with ulcerative colitis
    (BioMed Central Ltd, 2023) Buran T.; Batır M.B.; Çam F.S.; Kasap E.; Çöllü F.; Çelebi H.B.G.; Şahin M.
    Background: Ulcerative colitis (UC) is a chronic inflammatory bowel disease that develops due to the impaired immune response in genetically susceptible individuals, and its etiopathogenesis is not fully elucidated. IL-17 A is a cytokine that is produced by a type of immune cell called Th17 cells and is involved in the immune response and inflammation. On the other hand, ADAMTS-1, -4, and − 5 are enzymes that are involved in the breakdown of extracellular matrix proteins, including proteoglycans, which are important components of the intestinal wall. This study aimed to evaluate the relationship between interleukin 17 (IL-17 A) cytokine, which plays a role in the pathogenesis of ulcerative colitis, and the inflammation-controlled a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-1, -4, and − 5 protein members. Methods: Bowel tissue samples and blood serum from 51 patients with UC and 51 healthy controls were included in this study. mRNA expression levels of the ADAMTS-1, -4, -5, and IL-17 A were analyzed by RT-qPCR, and immunohistochemical analyses were performed to evaluate ADAMTS-1, -4, -5, and IL-17 A proteins in tissue samples. In addition, ELISA analysis determined serum levels of the ADAMTS-1, -4, -5, and IL-17 A. Results: RT-qPCR results reveal that the expression of ADAMTS-1, -4, -5, and IL-17 A genes in the UC tissue samples were significantly high according to the control tissue samples. Also, ADAMTS-1, -4, -5, and IL-17 A proteins revealed enhanced expression pattern UC groups according to the control. Also, ADAMTS-1, -4, -5, and IL-17 A protein showed cytoplasmic localization patterns in both control and UC groups. The serum levels of ADAMTS-1,-5, and IL-17 A were significantly higher in UC samples than in the control group. Conclusions: We observed a positive correlation between the ADAMTS-1, -5 and IL17A cytokine expression in UC samples. These results provide a new understanding of controlling crucial ADAMTS family protein members by IL-17 A cytokines with UC. © 2023, BioMed Central Ltd., part of Springer Nature.