Browsing by Author "Çakmak S."

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    Random/aligned electrospun PCL/PCL-collagen nanofibrous membranes: Comparison of neural differentiation of rat AdMSCs and BMSCs
    (Institute of Physics Publishing, 2012) Çapkin M.; Çakmak S.; Kurt F.Ö.; Gumusderelioglu M.; Şen B.H.; Türk B.T.; Deliloǧlu-Gürhan S.I.
    In this study, the aligned (A) and randomly oriented (R) polycaprolactone (PCL-A and PCL-R) and PCL/collagen (PCL/Col-A and PCL/Col-R) nanofibers were electrospun onto smooth PCL membranes (PCLMs) prepared by solvent casting. In order to investigate the effects of chemical composition and nanotopography of fibrous surfaces on proliferation and on neural differentiation of mesenchymal stem cells (MSCs), adipose and bone marrow-derived rat MSCs (AdMSCs and BMSCs) were cultivated in suitable media i.e. inducing medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF), and cell maintenance medium (CMM). BMSCs adhered and proliferated on all nanofibrous membranes more efficiently than AdMSCs. PCL/Col-A was found as the most convenient surface supporting proliferation in both cell types. Immunofluorescence staining indicated that BMSCs and AdMSCs are prone for differentiation to oligodendrocytes more than they differentiate to other neuronal cell types. PCL-A nanofibrous membranes supported differentiation of MSCs to O4+ (an oligodendrocytes surface antigen) cells in both culture media. The intensity of immunoreactivity of O4+ cells differentiated from BMSCs on PCL-A was highest when compared with the other groups (p < 0.001). Some BIII-T signed neural cells were investigated on PCL-A nanofibrous membranes, but the intensity of immunoreactivity was lower than that of O4+ cells. In conclusion, this study can be evaluated to establish the cell therapy strategies in neurodegenerative disorders, which are relevant to oligodendrocyte abstinence using BMSCs or AdMSCs on aligned nanofibrous membranes. © 2012 IOP Publishing Ltd.
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    Photostimulation of osteogenic differentiation on silk scaffolds by plasma arc light source
    (Springer London, 2018) Çakmak A.S.; Çakmak S.; Vatansever H.S.; Gümüşderelioğlu M.
    Low-level laser therapy (LLLT) has been used for more than 30 years to heal wounds. In recent years, LLLT or photostimulation has been indicated as an effective tool for regenerative and dental medicine by using monochromatic light. The aim of this study is to indicate the usability of plasma arc light source for bone regeneration. This is why we used polychromatic light source providing effective wavelengths in the range of 590–1500 nm for cellular response and investigated photostimulation effects on osteogenic differentiation of human mesenchymal stem cells (hMSCs) seeded on 3D silk scaffolds. Cellular responses were examined by using cell culture methods in terms of proliferation, differentiation, and morphological analyses. The results showed that photostimulation with a polychromatic light source (applied for 5 min from the 3rd day after seeding up to the 28th day in 2-day intervals with 92-mW/cm2 power from 10-cm distance to the cells) enhanced osteogenic differentiation of hMSCs according to higher alkaline phosphatase (ALP) activity, collagen and calcium content, osteogenic gene expressions, and matrix mineralization. In conclusion, we suggest that the plasma arc light source that was used here has a great potential for bone regeneration. © 2017, Springer-Verlag London Ltd., part of Springer Nature.
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    The effect of personal, familial, and environmental characteristics on acne vulgaris: A prospective, multicenter, case controlled study
    (Edizioni Minerva Medica, 2019) Karadag A.S.; Balta I.; Saricaoglu H.; Kiliç S.; Kelekçi K.H.; Yildirim M.; Arica D.A.; Ozturk S.; Karaman G.; Çerman A.A.; Bilgili S.G.; Turan E.; Demirci M.M.; Uzunçakmak T.K.; Guvenç S.C.; Ataseven A.; Ferahbaş A.; Aksoy B.; Çolgeçen E.; Ekiz O.; Demir F.T.; Bilgiç O.; Çakmak S.; Uçmak D.; Ozuguz P.; Konkuralp Y.K.; Ermertcan A.T.; Gokdemir G.; Başkan E.B.; Alyamaç G.; Şanli H.
    BACKGROUND: There are only a few studies about epidemiological features of acne vulgaris in the literature. The aim of this study was to analyze demographic, clinical, familial and environmental characteristics of acne, the role of diet and aggravating factors and association of these factors with acne severity. METHODS: Patients with a diagnosis of mild-moderate to severe acne were consecutively interviewed at the participating centers during the study period. RESULTS: A total of 3826 patients and 759 control patients were involved in this study. Mild acne was the most common type of acne, and most of the lesions were localized on face followed by the trunk. The severity of acne was worse in patients who had a positive family history of acne. The most common triggering factor was psychological stress. We found a positive correlation with chocolate, bread, green tea, milk, white sugar, ripe banana, ice cream, apple, orange, and red meat consumption. As we compare the acne severity according to geographical features we detected mild- moderate acne was more common in Mediterranean region and severe acne was more common in East Anatolian region. Family history positivity was more common in Aegean region and least common in Central Anatolian region. There was statistically significant relationship as we compare acne severity and dietary factors such as chocolate, dairy products such as milk, sunflower seed consumption within the geographical regions. CONCLUSIONS: This study presents the demographic and clinical characteristics of acne patients in Asian and the European parts of Turkey. We believe that this study will provide a useful overview of acne in Turkey. © 2017 EDIZIONI MINERVA MEDICA.