Browsing by Author "Çigel, A"

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    The effect of a single dose of Mk-801 use on adult brain tissue after an experimental head trauma model applied in immature rats
    Çigel, A; Sayin, O; Gürgen, SG; Sönmez, A
    Objective: Within the scope of this research, the long-term effects of experimental blunt head trauma on immature rats and MK-801 administered acutely after trauma on the brain tissue will be examined. In addition, the impact of trauma and MK-801 on Nestin and CD133, which are essential stem cells, will be evaluated by immunohistochemical and ELISA methods. Methods: In this study, the contusion trauma model was used. Sprague Dawley rats 30 7-day-old were divided into three groups: Group 1 (n = 10) control group, Group 2 (n = 10) trauma Group (head trauma applied), and Group 3 (n = 10) MK-801 + trauma Group. In the third group, immediately after head trauma, MK-801 (Sigma M107) dissolved in physiological saline was administered as a single dose of 1 mg/kg ip. Results: The concentration of nestin was significantly higher in the control group compared to both the trauma and trauma+drug groups (p < 0.001). CD133 was statistically significantly higher in the control group compared to the other two groups (p = 0.002). It was determined that the differences in Nestin CA1 and DG measurements resulted from the trauma and control and trauma and trauma+drug groups, and the differences in CD133 CA1 and DG measurements resulted from the trauma and control group. Conclusion: The positive effect of MK-801 on neuroprotective and neuronal proliferation was elaborated. Administration of MK-801 significantly induced nestin and CD133 concentrations in the injured tissue.
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    Does Microwave Exposure at Different Doses in the Pre/Postnatal Period Affect Growing Rat Bone Development?
    Karadayi, AA; Sarsmaz, HY; Çigel, A; Engiz, BK; Ünal, NA; Ürkmez, SS; Gürgen, SG
    Effects of pre/postnatal 2.45 GHz continuous wave (CW), Wireless -Fidelity (Wi-Fi) Microwave (MW) irradiation on bone have yet to be well defined. The present study used biochemical and histological methods to investigate effects on bone formation and resorption in the serum and the tibia bone tissues of growing rats exposed to MW irradiation during the pre/postnatal period. Six groups were created: one control group and five experimental groups subjected to low-level different electromagnetic fields (EMF) of growing male rats born from pregnant rats. During the experiment, the bodies of all five groups were exposed to 2.45 GHz CW-MW for one hour/day. EMF exposure started after fertilization in the experimental group. When the growing male rats were 45 days old in the postnatal period, the control and five experimental groups' growing male and maternal rats were sacrificed, and their tibia tissues were removed. Maternal rats were not included in the study. No differences were observed between the control and five experimental groups in Receptor Activator Nuclear factor-kB (RANK) biochemical results. In contrast, there was a statistically significant increase in soluble Receptor Activator of Nuclear factor-kB Ligand (sRANKL) and Osteoprotegerin (OPG) for 10 V/m and 15 V/m EMF values. Histologically, changes in the same groups supported biochemical results. These results indicate that pre/postnatal exposure to 2.45 GHz EMF at 10 and 15 V/m potentially affects bone development.