Browsing by Author "Çolak, H"

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    Evaluation of reticulocyte parameters in iron deficiency, vitamin B12 deficiency and β-thalassemia minor patients
    Ceylan, C; Miskioglu, M; Çolak, H; Kiliççiogly, B; Özdemir, E
    The aim of this study was to test the clinical utility of reticulocyte parameters in differential diagnosis in iron deficiency anemia (IDA), vitamin B-12 deficiency (B12) and beta-thalassemia minor (TM). We analyzed the percentage of reticulocyte, absolute reticulocyte count, mean content hemoglobin of reticulocyte (CHr), mean corpuscular volume of reticulocyte (MCVr), corpuscular hemoglobin concentration mean of reticulocyte (CHCMr), MCVr/MCV ratio, CHr/CH ratio and CHCMr/CHCM ratio in healthy donors (n = 34), iron deficiency (IDA) (n = 41), vitamin B-12 deficiency (B12) (n = 22), and TM (n = 34). This study demonstrates that the cutoff value of CHr was 25.7 as indicative of IDA (85.4% sensitivity, 97.1% specificity). CHr and MCVr may be useful for TM (cutoff value <= 24.8 for CHr) and B12 (> 102.1, cutoff value for MCVr), respectively. Sensitivity and specificity of these parameters were 90.9, 86.4% and 97.1, 82.4%, respectively. CHCMr is useful to differentiate IDA and TM from B12. While CHr was low value in microcytic groups (mean 21.8 +/- 3.3 for IDA, 21.0 +/- 2.9 for TM), it was high in B12 (mean 32.1 +/- 5.7). However, that of CHr/CH ratio was only significantly in IDA group compared with the control (P < 0.05, mean 0.98). Therefore, there are limitations regarding CHr and CHr/CH ratio differential diagnosis in microcytic and macrocytic groups. CHr, MCVr, and CHCMr are not sufficiently sensitive and specific to differentiate TM from IDA. We conclude that measurement of reticulocyte count and parameters may be a very useful implement in the diagnosis of IDA and TM.
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    Relationship Between Epicardial Adipose Tissue, Inflammation and Volume Markers in Hemodialysis and Transplant Patients
    Çolak, H; Kilicarslan, B; Tekce, H; Tanrisev, M; Tugmen, C; Aktas, G; Kursat, S
    Cardiovascular (CV) diseases are still the most important cause of morbidity and mortality in both patients receiving hemodialysis (HD) treatment and individuals with renal transplantation (Rtx). Measurement of epicardial adipose tissue (EAT) thickness is an easily applied, cheap, and useful recent method predicting increased CV risk. We aimed to compare EAT changes in HD and Rtx patients and the association between EAT and inflammatory and CV volume markers in both groups. A total of 124 patients: 45 Rtx, 43 HD patients and 36 healthy controls were enrolled in the study. Laboratory parameters and inflammatory markers (interleukin-6 [IL-6] and high sensitive C-reactive protein [Hs-CRP]) were evaluated from venous blood samples after an overnight fast. EAT thickness was measured with transthoracic echocardiography. The levels of Hs-CRP, IL-6, systolic and diastolic blood pressures, left atrial (LA) diameter, left atrial index (LAI), left ventricular mass (LVM) and LVM index (LVMI) were significantly higher in the HD patients than in the other groups. EAT was positively correlated with age, body mass index (BMI), time on dialysis, serum creatinine, total cholesterol, Low density lipoprotein-cholesterol, and LVM in Rtx group and positively correlated with age, BMI, duration of dialysis, Hs-CRP, IL-6, LAI and LVMI and inversely correlated with inferior vena cava collapse index (IVC-CI) in HD group. EAT thickness of RTx patients (whose previous HD duration was similar to those in HD group) are similar to the healthy population and significantly thinner than patients on HD.
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    Relationship Between Angiotensin-converting Enzyme Gene Polymorphism and QT Dispersion in Hemodialysis Patients
    Toraman, A; Çolak, H; Tekçe, H; Cam, S; Kürsat, S
    Introduction. The angiotensin-converting enzyme (ACE) gene insertion or deletion in long-term hemodialysis patients may be associated with corrected QT interval prolongation, leading to fatal arrhythmias. The ACE D allele is known to increase the risk of malignant ventricular arrhythmias and is also associated with increased QT dispersion after myocardial infarction and hypertension. This study aimed to evaluate the relationship between ACE gene polymorphism and QT dispersion in hemodialysis patients. Materials and Methods. In 70 hemodialysis patients, electrocardiography was performed and QT dispersion was calculated. Corrected QT interval was calculated using Bazett Formula. The ACE gene polymorphism was determined by polymerase chain reaction. Results. The mean age of the patients was 60 +/- 12 years. The mean QT dispersion and corrected QT dispersion were 61.71 +/- 21.99 and 73.18 +/- 25.51, respectively. QT dispersion inversely correlated with serum calcium and potassium levels and positively correlated with ACE gene polymorphism and residual urine. Calcium level was the predictor factor for QT dispersion. The ACE genotype correlated with QT dispersion, corrected QT dispersion, hemoglobin, and residual urine, and inversely correlated with serum potassium. Corrected QT dispersion correlated with ACE gene polymorphism and residual urine. The DD genotype of ACE had significally greater QT dispersion and corrected QT dispersion than the II and ID genotypes. Conclusions. Our study showed that the most important parameter affecting corrected QT dispersion was ACE gene polymorphism on the background of D allelle. Patients carrying this allelle need special attention regarding optimal suppression of renin-angiotensin-aldosteron system activity.
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    Correlation Between Intestinal Colony Numbers and Inflammation Markers (TNF-Alpha, IL-6, High-Sensitive CRP) Following the Use of Sevelamer in Rats with Kidney Failure
    Kürsat, S; Çolak, H; Yilmaz, O; Kolatan, E; Ulman, C; Kurutepe, S; Özyurt, B
    Objective: Our aim was to investigate anti-inflammatory effect of sevelamer in experimentally induced renal failure (RF) and whether this effect was related to a decrease in Escherichia coli colony counts in feces. Material and Methods: Eighteen female Wistar albino rats weighted 200-250 g were divided into three groups. RF was induced by 5/6 nephrectomy. Group 1 was the control group. Group 2 rats were those with induced RF receiving a high phosphate diet. Group 3 contained rats with induced RF receiving a high phosphate diet + sevelamer. E.coli count in feces, plasma creatinine, high-sensitive C-reactive protein (hsCRP), interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) concentrations were determined at the beginning and at the end of 1st, 3rd and 6th weeks in all rats. Results: In Group 2 and 3, creatinine concentrations were found to be increased (p=0.03, p=0.02) in the 1st week. In Group 2, there was an increase in number of E. coli colony count in the 3rd week (p=0.05). In group 3, E. coli colony count and levels of inflammatory markers (IL-6, CRP, TNF-alfa) significantly were increased in comparison to the basal :+alues (p=0.042, p=0.021, p=0.042, respectively) in the 1st week. In group 3, in the sixth week, E. coli colony count (p=0.02) and levels of inflammatory markers (IL-6, hsCRP, TNFalpha) (p<0.05) were found to be significantly decreased in comparison to the 1st week levels. Conclusion: Anti-inflammatory effects of sevelamer might be explained by its effects on the bacterial colonization in colon.