Browsing by Author "Özdal-Kurt, F"
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Item The cytotoxic, neurotoxic, apoptotic and antiproliferative activities of extracts of some marine algae on the MCF-7 cell lineKurt, O; Özdal-Kurt, F; Tuglu, MI; Akçora, CMWe investigated the cytotoxic, neurotoxic, apoptotic and antiproliferative effects of extracts from Petalonia fascia, Jania longifurca and Halimeda tuna on the MCF-7 breast cancer cell line. J. longifurca extracts were more toxic than those of P. fascia and H. tuna. The algal extracts showed significant toxic effects at different dilutions. The toxic effects were due to increased oxidative stress and resulted in apoptosis. Algal toxicity may exert negative effects through the food chain or by direct interaction. Algal toxicity also has potential for cancer therapy. The toxic effects that we observed may be especially important for therapy for breast tumors.Item The effect of osteogenic medium on the adhesion of rat bone marrow stromal cell to the hydroxyapatiteDeliloglu-Gurhan, I; Tuglu, I; Vatansever, HS; Özdal-Kurt, F; Ekren, H; Taylan, M; Sen, BHObjective: To investigate the adhesive properties of bone marrow stromal cell (BMSC) on the hydroxyapatite (HA) particles and analyze their behavior. Methods: The study took place in the Department of the Histology and Embryology, Celal Bayar University, Manisa and in the Department of Bioengineering, Ege University, Izmir, Turkey between 2004 and 2005. We cultured BMSC from the mature rat tibia and differentiated to the osteoblasts by osteogenic medium. The BMSCs were subcultured and were taken to the HA substrate. We measured their proliferation capacity and viability with MTT assay using the spectrophotometric method. Furthermore, we identified the osteoblast-like cells by immunohistochemical staining of osteonectin and osteocalcin and we analyzed the behavior of the cells on different sized HA particles by SEM at the end of 3 days incubation. Results: Osteogenic medium caused the proliferation capacity of BMSC to speed up and the effects appeared earlier. We confirmed the osteoblastic differentiation by staining of most cells with osteoblastic markers. Subcultured cells were similarly adhesive to the HA particles and the osteogenic medium did not alter this behavior. They spread on the substrate similarly. Most of the cells demonstrated the cytoplasmic protrusion. Morphology of the cells did not change much with or without osteogenic medium. Different sizes of HA particles did not affect the adhesive properties of these cells except HA gel. The spreading and attachment ratios of the cells on HA gel were more than the others. Conclusion: We found that there was heterogeneity in BMSC on differentiation capacity to the osteoblast, which was a sign of a subpopulation. Adhesive cells showed similar morphology and behavior under the effect of osteogenic medium. The only difference was the spreading capacity on the HA gel where cell used this substrate more effectively for adhesion.Item The Contribution of Differentiated Bone Marrow Stromal Stem Cell-Loaded Biomaterial to Treatment in Critical Size Defect Model in RatsTuglu, MI; Özdal-Kurt, F; Koca, H; Sarac, A; Barut, T; Kazanç, AMandibular fractures present a challenge in maxillo-facial surgery due to difficulties in healing and complications. In recent years, advances in bio-engineering as well as stem-cell studies suggest that it may be possible to treat these fractures by stem cell treatment with biomaterials. In the present study, we explored the efficacy of differentiated stem cells placed on biomaterials on fracture treatment and its relation with oxidative stress and apoptosis. A 4 mm circular defect was made on the mandibulae of 20 adults Wistar rats. Hydroxyapatite gel (control, n=5) and bone marrow stromal cells differentiated into osteoblast-seeded hydroxyapatite gel (n=5) were implanted within these defects. We were also used empty cavities (n=5) and cavities filled with only cells (n=5) for negative controls. Animals were sacrificed after a 6-week healing period and samples were examined blindly by histological, immunohistochemical, radiological and morphometric methods. Compared to the control cavities that underwent no procedure or filled with just cells, there were significant (P<0.001) healings in both groups. Hydroxyapatite gel with differentiated stem cells on, however, yielded significantly (P<0.05) better new bone formation and osteoid production decreased fibrous tissue and increased cellular activity. Differentiated stromal cells combined with biomaterial accelerated the treatment in defects of critical volume within a 6-week period of healing, activated and resulted in significant formation of bone of higher quality. Promotion of bone formation by the helps of bioengineering and stromal cells has gained importance in the treatment and reconstruction of fractures.Item In vivo evaluation of cerium, gallium and vanadium-doped borate-based bioactive glass scaffolds using rat subcutaneous implantation modelDeliormanli, AM; Vatansever, HS; Yesil, H; Özdal-Kurt, FThe main objective of this study was to evaluate the cerium, gallium and vanadium-containing bioactive borate glass scaffolds for soft tissue applications and determine the potential toxicity of these scaffolds on the adjacent tissues. The effects of the cerium, gallium and vanadium substitution on the soft tissue ingrowth and angiogenesis in porous borate based bioactive glass scaffolds were investigated using rat subcutaneous implantation model. For this purpose, bioactive borate glass powders containing therapeutic ions were prepared by melt-cast method and subsequently scaffolds were fabricated using polymer foam replication technique. The scaffolds were implanted subcutaneously for 4 weeks in Sprague Dawley rats. Bare borate glass scaffolds with the same microstructure were used as the control. Histology was used to evaluate tissue ingrowth and blood vessel formation in the implants. Additionally, the antibacterial activities of cerium, gallium and vanadium containing porous bioactive glass scaffolds were investigated in vitro by a zone inhibition method. Results revealed that addition of cerium ions to the borate glass network caused an increase in blood vessel formation. On the other hand, a decrease was obtained in angiogenesis in gallium and vanadium-containing glasses. All of the scaffolds prepared in the study did not show any antibacterial activity towards Escherichia coli and Staphylococcus aureus. (C) 2016 Elsevier Ltd and Techna Group S.r.l. All rights reserved.Item Attachment and growth of dental pulp stem cells on dentin in presence of extra calciumÖzdal-Kurt, F; Sen, BH; Tuglu, I; Vatansever, S; Türk, BT; Deliloglu-Gürhan, IObjective: We aimed to differentiate dental pulp stem cells (DPSC) to odontoblast-like cells (ODPSC) and to investigate their attachment and growth on dentin in the presence of extra calcium by colorimetric assay and scanning electron microscopy (SEM). Methods: After isolation of DPSC, they were differentiated to ODPSC. Standard dentin discs from human molar teeth were prepared. While the dentin discs in Group 1 did not receive any extra treatment, the discs in Group 2 were treated with acidic calcium phosphate precipitation (CPP) solution. In Group 3, the discs were suspended in phosphate buffered saline containing calcium. DPSC or ODPSC (3 x 10(4) cells/mL) were seeded on all discs and incubated for 7,14 or 21 days. Attachment and growth of 7-day cell cultures on extra dentin samples were examined by SEM. MTT assay showed that number of cells on dentin surfaces was increased by time periods regardless of type of treatment and cells (p < 0.05). Results: While DPSC and ODPSC showed similar proliferation rates at 7 and 14 days (p > 0.05), the number of ODPSC was higher than DPSC in 21-day samples (p = 0.039). MTT assay showed that number of cells on dentin surfaces was increased by time periods regardless of type of treatment and cells (p < 0.05). Calcium-treated dentin surfaces always had lower number of cells; being significant for only CPP-treated surfaces (p < 0.01). Both types of cells demonstrated good attachment and proliferation on dentin surfaces regardless of type of dentin treatment. Conclusions: Because the nature of dentin surface itself showed good adhesive characteristics with ODPSC and DPSC, additional calcium treatment of dentin surfaces may not be necessary. (C) 2016 Published by Elsevier Ltd.Item Enhanced biocompatibility of GPC by ion implantation and depositionZimmerman, R; Gürhan, I; Muntele, C; Ila, D; Rodrigues, M; Özdal-Kurt, F; Sen, BHBiocompatible Glassy Polymeric Carbon (GPC) is used for artificial heart valves and in other biomedical applications. Although it is ideally suited for implants in the blood stream, tissue that normally forms around the moving parts of a GPC heart valve sometimes loses adhesion and creates embolisms downstream. We have previously shown that oxygen ion implantation slightly enhances cell adhesion to GPC. Here we compare silver ion implantation and silver deposition, each of which strongly inhibits cell attachment on GPC. Inhibition of cell adhesion is the more desirable improvement to current GPC cardiac implants. In vitro biocompatibility tests have been carried out with model cell lines to demonstrate that traces of silver can favorably influence the surface of GPC for biomedical applications. (c) 2007 Published by Elsevier B.V.Item Neurotoxic, cytotoxic, apoptotic and antiproliferative effects of some marine algae extracts on the NA2B cell lineKurt, O; Özdal-Kurt, F; Akçora, CM; Özkut, M; Tuglu, MIOxidative stress contributes to cancer pathologies and to apoptosis. Marine algae exhibit cytotoxic, antiproliferative and apoptotic effects; their metabolites have been used to treat many types of cancer. We investigated in culture extracts of Petalonia fascia, Jania longifurca and Halimeda tuna to determine their effects on mouse neuroblastoma cell line, NA2B. NA2B cells were treated with algae extracts, and the survival and proliferation of NA2B cells were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effects of algae extracts on oxidative stress in NA2B cells also were investigated using nitric oxide synthase (NOS) immunocytochemistry and apoptosis was assessed using terminal deoxynucleotidyl transferase dUTP nick end labeling. We observed significant neurite inhibition with moderate damage by the neurotoxicity-screening test (NST) at IC50 dilutions of the extracts. MTT demonstrated that J. longifurca extracts were more toxic than P. fascia and H. tuna extracts. We found an increase of endothelial and inducible NOS immunostaining for oxidative stress and TUNEL analysis revealed increased apoptosis after application of extract. Our findings suggest that the algae we tested may have potential use for treatment of cancer.Item The effect of autologous bone marrow stromal cells differentiated on scaffolds for canine tibial bone reconstructionÖzdal-Kurt, F; Tuglu, I; Vatansever, HS; Tong, S; Deliloglu-Gürhan, SIBone marrow contains mesenchymal stem cells that form many tissues. Various scaffolds are available for bone reconstruction by tissue engineering. Osteoblastic differentiated bone marrow stromal cells (BMSC) promote osteogenesis on scaffolds and stimulate bone regeneration. We investigated the use of cultured autologous BMSC on different scaffolds for healing defects in tibias of adult male canines. BMSC were isolated from canine humerus bone marrow, differentiated into osteoblasts in culture and loaded onto porous ceramic scaffolds including hydroxyapatite 1, hydroxyapatite gel and calcium phosphate. Osteoblast differentiation was verified by osteonectine and osteocalcine immunocytochemistry. The scaffolds with stromal cells were implanted in the tibial defect. Scaffolds without stromal cells were used as controls. Sections from the defects were processed for histological, ultrastructural, immunohistochemical and histomorphometric analyses to analyze the healing of the defects. BMSC were spread, allowed to proliferate and differentiate to osteoblasts as shown by alizarin red histochemistry, and osteocalcine and osteonectine immunostaining. Scanning electron microscopy showed that BMSC on the scaffolds were more active and adhesive to the calcium phosphate scaffold compared to the others. Macroscopic bone formation was observed in all groups, but scaffolds with stromal cells produced significantly better results. Bone healing occurred earlier and faster with stromal cells on the calcium phosphate scaffold and produced more callus compared to other scaffolds. Tissue healing and osteoblastic marker expression also were better with stromal cells on the scaffolds. Increased trabecula formation, cell density and decreased fibrosis were observed in the calcium phosphate scaffold with stromal cells. Autologous cultured stromal cells on the scaffolds were useful for healing of canine tibial bone defects. The calcium phosphate scaffold was the best for both cell differentiation in vitro and bone regeneration in vivo. It may be possible to improve healing of bone defects in humans using stem cells from bone marrow.Item The effect of different implant biomaterials on the behavior of canine bone marrow stromal cells during their differentiation into osteoblastsÖzdal-Kurt, F; Tuglu, I; Vatansever, HS; Tong, S; Sen, BH; Deliloglu-Gürhan, SIWe investigated the effects of different implant biomaterials on cultured canine bone marrow stromal cells (BMSC) undergoing differentiation into osteoblasts (dBMSC). BMSC were isolated from canine humerus by marrow aspiration, cultured and differentiated on calcium phosphate scaffold (CPS), hydroxyapatite, hydroxyapatite in gel form and titanium mesh. We used the MTT method to determine the effects of osteogenic media on proliferation. The characteristics of dBMSC were assessed using alizarin red (AR), immunocytochemistry and osteoblastic markers including alkaline phosphatase/von Kossa (ALP/VK), osteocalcin (OC) and osteonectin (ON), and ELISA. The morphology of dBMSC on the biomaterials was investigated using inverted phase contrast microscopy and scanning electron microscopy. We detected expression of ALP/VK, AR, OC and ON by day 7 of culture; expression increased from day 14 until day 21. CPS supported the best adhesion, cell spreading, proliferation and differentiation of BMSCs. The effects of the biomaterials depended on their surface properties. Expression of osteoblastic markers showed that canine dBMSCs became functional osteoblasts. Tissue engineered stem cells can be useful clinically for autologous implants for treating bone wounds.Item Cell adhesion study of the titanium alloys exposed to glow dischargeAbidzina, V; Deliloglu-Gürhan, I; Özdal-Kurt, F; Sen, BH; Tereshko, I; Elkin, I; Budak, S; Muntele, C; Ila, DTitanium for biomedical application stems mainly from its advantageous bulk mechanical properties in combination with a high degree of biocompatibility that is largely attributable to their surface properties. This work is focused on the investigation of surface properties of treated titanium and cell adhesion to titanium treated in glow-discharge plasma. Pure titanium samples (grade 4) were exposed to low-energy ion irradiation in a specially constructed plasma generator, where materials were irradiated by ions of residual gases in vacuum. The ion energy was 1-10 keV. The irradiation dose was maintained at 10(17) ions cm(-2). The irradiation time varied from 5 to 60 min. Rutherford backscattering spectrometry (RBS) was used for surface studies. RBS showed the presence of iron on the titanium surface that occurred from the cathode of plasma generator. In vitro biocompatibility test have been carried out with model cell lines (L929 mouse fibroblasts) to demonstrate that low-energy ion irradiation can favorably influence the surface of titanium for biomedical application. Scanning electron microscopy (SEM) was the main tool to demonstrate the cell attachment properties. (c) 2007 Elsevier B.V. All rights reserved.