Browsing by Author "Özensoy Töz S."

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    Seroprevalence of canine visceral leishmaniasis in Kuşadasi, Turkey
    (TUBITAK, 2005) Özensoy Töz S.; Ertabaklar H.; Özbel Y.; Cüneyt Balcioǧlu I.; Yildizli N.; Alkan M.Z.
    Human and canine visceral leishmaniasis cases have been reported from Kuşadasi, a town in western Turkey, since 1993. In orcer to better understand the epidemiology of canine visceral leishmaniasis (CanVL) in the region, we aimed: (i) to determine the prevalence of CanVL in Kuşadasi using a randomly selected dog population and (ii) to measure the effect of case control on the prevalence of the disease. In this study, all 109 dogs kept at the dog shelter of Kuşadasi Municipality (reflecting random selectivity) were screened serologically using IFAT and rK39 ELISA in 1999. Ten dogs (9.1%) were seropositive or borderline in 1 of the 2 tests. Seropositive dogs (8) died spontaneously or were euthanized, while borderline dogs (2) underwent monthly serological examinations. One year later, in a second sampling, a total of 85 dogs were examined (27 previously tested and 58 new). Four dogs 4.7%) showed seropositivity. Seropositive dogs (2) were euthanized while borderline dogs (2) underwent monthly serological examinations. The reduction in the prevalence of the disease (from 9.1% to 4.7%) appears to be the result of culling CanVL positive dogs from the general population. © TÜBİTAK.
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    A new approach for determining the spatial risk levels for visceral and cutaneous leishmaniasis related with the distribution of vector species in western part of turkey using geographical information systems and remote sensing; [Coğrafi bilgi sistemleri ve uzaktan algılama kullanılarak türkiye’nin batısında visseral ve kutanöz leishmaniasisde vektör türlerin dağılımı ile ilişkili mekansal risk düzeylerinin saptanması için yeni bir yaklaşım]
    (Veteriner Fakultesi Dergisi, 2012) Ölgen M.K.; Özbel Y.; Balcioğlu I.C.; Demir S.; Şimşek F.; Özensoy Töz S.; Ertabaklar H.; Alkan M.Z.
    Leishmaniases are present in two clinical forms, as visceral and cutaneous, in Turkey showing a tendency of spreading throughout the country. The aim of the present study was to produce a new model for determining the spatial risk levels using the data in a selected study site in the western part of Turkey. The results of entomological studies in this leishmaniasis focus indicated the presence of suspected vector species Phlebotomus (Larroussius) tobbi and P. (Larroussius) neglectus for the visceral, P. (Paraphlebotomus) similis for cutaneous forms of the disease. The new risk model was developed based on univariate and multivariate binary logistic regression analyses of geographical variables as altitude, aspect, Normalized Difference Vegetation Index (NDVI), Enhanced Vegetation Index (EVI) and Land Surface Temperature (LST) values related to the distribution of these three species. The results of the new model were used to produce the risk maps and the potential distribution areas of the incriminated vector species with the use of geographical technologies which allowed the identification of the leishmaniasis risk levels that may provide useful information to guide the control program interventions. © 2012, Veteriner Fakultesi Dergisi. All rights reserved.
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    The comparison of parasitological and bacteriological stool examinations in patients with gastrointestinal symptoms; [Gastrointestinal sistem yakınması olan hastalarda dışkının parazitolojik ve bakteriyolojik incelemelerinin karşılaştırılması]
    (Veteriner Fakultesi Dergisi, 2012) Bayram A.; Oyur T.; Ünver A.; Aydemir Ş.; Özacar T.; Özensoy Töz S.; Turgay N.
    Stool samples of 236 patients with acute and chronic gastrointestinal symptoms admitted to Ege University Medical Faculty Parasitology Outpatient Clinic Laboratory between July 2009 to June 2010 were examined. One hundred eleven out of 236 (47%) patients admitted to the laboratory during summer and autumn period with acute gastrointestinal symptoms while other 125 patients (53%) admitted during winter and spring with chronic symptoms. At least one parasite was determined in 112 out of 236 (47.45%) patients. In retrospective analysis, it has determined that the bacteriological examination of stool samples were also performed in 121 out of 236 (51.7%) patients. Seven out of 121 (5.78%) patients admitted both bacteriological and parasitological laboratories together had at least one bacterial agent in their stool examination. Five out of 7 patients were found to be having bacteriological and parasitological mixed infection. Our findings showed that it is important to perform both bacteriological and parasitological examinations together in patients admitting to hospital with intestinal symptoms due to these mixed infections. Performing both diagnostic techniques together will improve accurate diagnosis, treatment and understanding possible etiological reasons of these mixed infections. © 2012, Veteriner Fakultesi Dergisi. All rights reserved.
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    E.Histolytica/dispar cases diagnosed in ege university medical faculty parasitology outpatient clinic between January 2010 to June 2011; [Ocak 2010-haziran 2011 tarihleri arasında ege üniversitesi tıp fakültesi parazitoloji polikliniğinde saptanan e. Histolytica/dispar olguları]
    (Veteriner Fakultesi Dergisi, 2012) Ünver A.; Oyur T.; Kurt Ö.; Özensoy Töz S.; Turgay N.
    For definitive diagnosis of amoebiasis, The Ministry of Health requires the detection of Entamoeba histolytica (E. histolytica) trophozoites that ingested red blood cells in Trichrome-stained smears or E. histolytica-specific adhesin antigen with ELISA. Stool samples of 51 patients admitted to the Outpatients Clinic of Ege University School of Medicine Department of Parasitology between January 2010 and June 2011 were suspected to have E. histolytica/dispar cysts or trophozoites in wet mount examinations and stained with Trichrome. Examination of these smears revealed that 49 samples were positive for E. histolytica/dispar Thirty-three samples were tested for the positivity of E. histolytica-specific adhesin antigen with a commercial ELISA kit (Entamoeba CELISA-Path; CeLLabs Pty. Ltd., Brookvale, Australia) and 23 were found to be positive. Our results indicated an elevation of figures of amoebiasis cases in recent years. It is concluded that application of different methods for the diagnosis of E. histolytica infections as suggested by The Ministry of Health is essential for correct reports of peripheral laboratories. © 2012, Veteriner Fakultesi Dergisi. All rights reserved.
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    Leishmaniasis in Turkey: Determination of Leishmania species by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)
    (Tehran University of Medical Sciences (TUMS), 2014) Culha G.; Akyar I.; Yildiz Zeyrek F.; Kurt Ö.; Gündüz C.; Özensoy Töz S.; Östan I.; Cavus I.; Gülkan B.; Kocagöz T.; Özbel Y.; Özbilgin A.
    Background: Cutaneous leishmaniasis (CL) is endemic in Southeastern Anatolia, mainly in Sanliurfa and Hatay provinces, and the causative agents are mostly Leishmania tropica and less frequently L. infantum. Here, we report the first MALDI-TOF analyses of Leishmania promastigotes obtained from the cultures of two CL cases from Osmaniye and Hatay provinces who were initially diagnosed by microscopy, culture and identified as L. infantum with Real-Time PCR (RT-PCR). Methods: Samples obtained from the skin lesions of patients were initially stained with Giemsa and cultivated in NNN medium. Examination of the smears and cultures revealed Leishmania amastigotes and promastigotes, respectively. The promastigotes (MHOM/TR/2012/CBU15 and MHOM/TR/2012/MK05) obtained from the cultures of both patients were used for RT-PCR targeting the ITS-1 region in the SSU of rRNA. The reference strains of four Leishmania species (L. infantum, L. donovani, L. tropica and L. major) were initially assessed with MALDI-TOF and their data were added to MALDI-TOF Biotyper Library. Results: Both RT-PCR and MALDI-TOF analyses indicated that the causative agent in both patient samples was L. infantum. Conclusion: Despite disadvantages such as requirement of culture fluid with nothing but promastigotes and high cost, MALDI-TOF analysis may be a fast, sensitive and specific diagnostic tool in especially large-scale research studies, where the cost declines, relatively.