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  1. Home
  2. Browse by Author

Browsing by Author "Özkan Ö."

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    Determination of the median lethal dose and electrophoretic pattern of Hottentotta saulcyi (Scorpiones, Buthidae) scorpion venom
    (Tehran University of Medical Sciences, 2015) Yağmur E.A.; Özkan Ö.; Karaer K.Z.
    Background: In this study, we investigated the lethal potency, electrophoretic protein pattern and in vivo effects of Hottentotta saulcyi scorpion venom in mice. Methods: Scorpions were collected at night, by using a UV lamp from Mardin Province, Turkey. Venom was obtained from mature H. saulcyi scorpions by electrical stimulation of the telson. The lethality of the venom was determined by i.v. injections using Swiss mice. In vivo effects of the venom were assessed by using the intraperitoneal route (ip) injections into mice (20±1g) and monitored for 24 h. The protein profiles of the scorpion venom were analyzed by NuPAGE® Novex® 4-12 % gradient Bis-Tris gel followed by Coomassie blue staining. Results: The lethal assay of the venom was 0.73 mg/kg in mice. We determined the electrophoretic protein pattern of this scorpion venom to be 4, 6, 9, 31, 35, 40, 46 and 69 kDa by SDS-PAGE. Analysis of electrophoresis indicated that H. saulcyi scorpion intoxicated mice exhibited autonomic nervous system symptoms (tachypnea, restlessness, hyperexcitability, convulsions, salivation, lacrimation, weakness). Conclusions: Hottentotta saulcyi scorpion venom includes short-chain neurotoxins and long-chain neurotoxins according to the electrophoretic protein patterns. The stings of H. saulcyi scorpion must be considered of risk for humans in the southeastern region, Turkey.
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    Phylogenetic relationships among the Leiurus abdullahbayrami (Scorpiones: Buthidae) populations in Turkey inferred from mitochondrial DNA sequence data
    (Universitatea din Oradea, 2018) Ayhan H.; Atay E.; Çarhan A.; Yağmur E.A.; Özkan Ö.; Çelebi B.
    Mitochondrial DNA (mtDNA) has been widely employed in phylogenetic studies. Leiurus abdullahbayrami was recognised has representing a different Leiurus scorpion species in 2009, with several morphological characters that differentiate it from Leiurus quinquestriatus, the genus type species. In this study, Leiurus abdullahbayrami samples collected from the provinces of Hatay, Kilis, Gaziantep, Şanliurfa, Adiyaman, and Mardin in Turkey were used as study material. The genetic identification of the L. abdullahbayrami (Scorpiones: Buthidae) was carried out by using the 16S mitochondrial gene, subunit of mtDNA. According to the results of this study, the little genetic difference between the groups was confirmed and the taxonomic validity of Leiurus abdullahbayrami was confirmed with molecular data. The 16S rRNA sequence data showed that the genus Leiurus seems to be represented in Turkey by a single species, L. abdullahbayrami. Nucleotide variations found in the current work constitute the first descriptive report for L. abdullahbayrami. ©Biharean Biologist, Oradea, Romania, 2018
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    Generations, genders and priorities: On digital and printed newspapers
    (Peter Lang AG, 2020) Özkan Ö.
    [No abstract available]

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