Browsing by Author "Öztürk, VÖ"

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    Gingival crevicular fluid interleukin-36β (-1F8), interleukin-36γ (-1F9) and interleukin-33 (-1F11) levels in different periodontal disease
    (PERGAMON-ELSEVIER SCIENCE LTD) Kursunlu, SF; Öztürk, VÖ; Han, B; Atmaca, H; Emingil, G
    Background: Periodontal inflammation is driven by the coordinated action of a number of factors, including the IL-1 family. Our study aimed to examine the levels of interleuldn (IL)-36 beta, IL-36 gamma and IL-33 levels in gingival crevicular fluid (GCF) from patients with different periodontal diseases. Materials and methods: A total of 80 subjects, 20 patients with generalized aggressive periodontitis (G-AgP), 20 patients with chronic periodontitis (CP), 20 with gingivitis and 20 periodontally healthy subjects were included. Periodontal status was evaluated by measuring probing depth, clinical attachment loss, papillary bleeding index and plaque index. GCF cytokine levels were analysed by ELISA. Results: CP, gingivitis and healthy groups had similar GCF IL-36 beta total amount (p > 0.008). G-AgP group had elevated IL-36 beta total amount compared to CP group (p < 0.008). G-AgP group had similar GCF IL-36 beta total amount to gingivitis and healthy groups (p > 0.008). GCF IL-36 gamma and IL-33 total amounts of the study groups were similar (p > 0.05). Conclusions: The present study demonstrated for the first time the presence of IL-36 beta, IL-36 gamma and IL-33 GCF levels with different periodontal diseases. High levels of IL-36-beta in the AgP group in comparison to CP group might suggest that periodontitis in the aggressive form could be related to the increase in GCF IL-36 beta. (C) 2014 Elsevier Ltd. All rights reserved.
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    Interleukin-6 Family of Cytokines in Crevicular Fluid of Renal Transplant Recipients With and Without Cyclosporine A-Induced Gingival Overgrowth
    (WILEY) Gürkan, A; Becerik, S; Öztürk, VÖ; Atmaca, H; Atilla, G; Emingil, G
    Background: Interleukin (IL)-6 family of cytokines, including IL-6, oncostatin M (OSM), leukemia inhibitory factor (LIF), and IL-11, have fibrogenic features. The current study determines gingival crevicular fluid (GCF) levels of fibrosis-related IL-6-type cytokines in cyclosporine A (CsA)-induced gingival overgrowth (GO). Methods: Eighty non-smokers were included (40 CsA-medicated renal transplant patients with GO [GO+; n = 20] or without GO [GO-; n = 20], 20 individuals with gingivitis, and 20 healthy participants). Probing depth and plaque, papilla bleeding, and hyperplastic index scores were recorded. GCF samples were obtained from the mesio-buccal aspects of two teeth. GCF IL-6, IL-1 beta, OSM, LIF, and IL-11 levels were analyzed by enzyme-linked immunosorbent assay. Results: The GO+ and GO- groups had higher IL-6 total amounts than the healthy group (P < 0.008). IL-1 beta total amounts in the GO+ group were significantly higher than in both the healthy and GO- groups (P < 0.008). OSM total amount was elevated in the GO+ and GO- groups compared with both the gingivitis and healthy groups (P < 0.008). All groups had similar LIF and IL-11 total amounts (P > 0.008). Moderate positive correlations were detected among IL-6, IL-1 beta, OSM, and IL-11 total amount in GCF and clinical parameters (P < 0.05). Conclusions: IL-6 and OSM increases in GCF as a result of CsA usage or an immunosuppressed state irrespective of the severity of inflammation and the presence of GO. The IL-6 family of cytokines might not be directly involved in biologic mechanisms associated with CsA-induced GO. Lack of an association between assessed IL-6 cytokines and CsA-induced GO might indicate distinct effects of these cytokines on fibrotic changes of different tissues.
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    Gingival crevicular fluid and salivary HIF-1α, VEGF, and TNF-α levels in periodontal health and disease
    (WILEY) Afacan, B; Öztürk, VÖ; Pasali, Ç; Bozkurt, E; Köse, T; Emingil, G
    Background Hypoxia-inducible factor-1 alpha (HIF-1 alpha) is expressed as an adaptive response to hypoxia, mediates angiogenesis through the expression of vascular endothelial growth factor (VEGF) and can be induced by tumor necrosis factor-alpha (TNF-alpha). This study aimed to investigate the gingival crevicular fluid (GCF) and salivary HIF-1 alpha, VEGF, and TNF-alpha levels in periodontal health and disease. Methods A total of 87 individuals, 20 generalized aggressive periodontitis (G-AgP), 20 chronic periodontitis (CP), 26 gingivitis patients, and 21 periodontally healthy individuals, were included. Clinical periodontal parameters were recorded; GCF and salivary samples were collected; and HIF-1 alpha, VEGF, and TNF-alpha levels were measured by enzyme-linked immunosorbent assay. Nonparametric tests were used for the statistical analyses. Results G-AgP and CP groups had significantly higher GCF HIF-1 alpha, VEGF, and TNF-alpha total amounts than gingivitis and healthy groups (P < 0.05). GCF HIF-1 alpha and TNF-alpha total amounts in gingivitis group were significantly higher than the healthy group (P < 0.05). GCF and salivary concentrations of biomarkers were similar in both periodontitis groups (P > 0.05). Salivary HIF-1 alpha concentrations in gingivitis group were significantly higher than G-AgP and healthy groups (P < 0.05). GCF HIF-1 alpha, VEGF, and TNF-alpha total amounts were positively correlated with the site-specific clinical periodontal parameters and with each other (P < 0.05). Conclusions HIF-1 alpha is detectable in GCF and saliva of periodontally diseased and healthy individuals, and the GCF levels of the biomarker can be affected by disease status. Increased GCF HIF-1 alpha, VEGF, and TNF-alpha levels in both chronic and aggressive form of periodontitis might suggest the role of TNF-alpha/HIF-1 alpha/VEGF pathway in the pathogenesis of periodontal diseases.
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    Gingival crevicular fluid calprotectin, osteocalcin and cross-linked N-terminal telopeptid levels in health and different periodontal diseases
    (HINDAWI LTD) Becerik, S; Afacan, B; Öztürk, VÖ; Atmaca, H; Emingil, G
    Aim: The aim of the present study was to investigate gingival crevicular fluid (GCF) calprotectin, osteocalcin and cross-linked N-terminal telopeptide (NTx) levels in health along with different periodontal diseases. Material and methods: Twenty chronic periodontitis (CP), 20 generalized aggressive periodontitis (G-AgP), 20 gingivitis and 20 healthy subjects were included. Probing depth, clinical attachment level, plaque index and papillary bleeding index was recorded. GCF calprotectin, osteocalcin and NTx levels were analyzed by enzyme-linked immunosorbent assay (ELISA). Results: CP, G-AgP and gingivitis groups had higher GCF calprotectin total amount compared to healthy subjects (p < 0.008). CP and G-AgP groups had similar, but higher levels compared to gingivitis groups (p < 0.008). CP and G-AgP groups had lower GCF osteocalcin total amount compared to gingivitis and healthy groups (p < 0.008). CP group had higher GCF NTx but lower osteocalcin total amount and osteocalcin/NTx ratio than the G-AgP group (p < 0.008). Conclusions: Our results suggest that elevated GCF calprotectin levels play a role as a reliable inflammatory marker in the pathogenesis of periodontal disease. Fluctuating GCF levels of osteocalcin and NTx might point out to the abnormal bone turnover in periodontitis. Our data document for the first time the role of NTx in the pathogenesis of different periodontal diseases.
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    Gingival Crevicular Fluid and Plasma Acute-Phase Cytokine Levels in Different Periodontal Diseases
    (WILEY) Becerik, S; Öztürk, VÖ; Atmaca, H; Atilla, G; Emingil, G
    Background: The aim of the present study is to investigate gingival crevicular fluid (GCF) and plasma acute-phase cytokines, interleukin-I beta (IL-1 beta), interleukin-6 (IL-6), interleukin-11 (IL-11), oncostatin M (OSM), and leukemia inhibitory factor (LIF) levels in patients with different periodontal diseases. Methods: Eighty individuals were included in this study; 20 with chronic periodontitis (CP), 20 with generalized aggressive periodontitis (GAgP), 20 with gingivitis, and 20 classified as healthy (H). Probing depth, clinical attachment level, plaque index, and papilla bleeding index were recorded. Plasma and GCF IL-1 beta, IL-6, IL-11, OSM, and LIF levels were analyzed by enzyme-linked immunosorbent assay. Results: CP and GAgP groups had significantly higher GCF IL-1 beta, IL-6, and IL-11 levels when compared with the H group (P <0.05). Conversely, GCF LIF levels of the CP and GAgP groups were lower than those of the H group (P<0.05). GCF OSM levels did not differ significantly among study groups. Plasma levels of all the cytokines studied were not significantly different among the study groups. Conclusions: Based on the present data, elevated IL-1 beta, IL-6, and IL-11 GCF levels, but not plasma levels, are suggested as reliable inflammatory biomarkers in periodontal diseases. Decreased LIF levels in diseased groups might reflect the possible beneficial effects of LIF in the modulation of inflammatory response in gingiva. J Periodontol 2012;83: 1304-1313.